Johns Hopkins University

The Lubrizol Corporation

Peter Schofield

Kieran F Scott

John M Watson

Barry G Rolfe

John Shine

Betty Eipper

Henry T Keutmann

Richard Mains

Henry Rodriguez

The sequence of bovine PAM is taught as well as new forms of PAM not known before. One new form is membrane bound and provides the basis of methods for alpha-amidating inactive precursors of peptide hormones.

cDNA encoding peptidyl-glycine alpha-amidating monooxygenase (PAM)

This invention provides useful promoters from the R. trifolii nifH gene for the construction of recombinant molecules to regulate foreign genes for expression under desired conditions. In particular, the promoters act to control expression of the foreign genes within root nodules formed by rhizobial bacterial strains in symbiotic combination with host plants. A rhizobium diagnostic segment (RDS) is also provided comprising a DNA segment found at more than one location in rhizobia, the RDS being species-specific, and detectable by DNA hybridization under stringent conditions. A recombinant plasmid comprising a RDS and a bacterial strain containing the plasmid are provided. Methods are provided for identifying species and strains of field isolates of Rhizobium, using RDS's. One RDS exemplified comprises 5' sequences from the R. trifolii nifH gene.

Rhizobial diagnostic probes and rhizobium trifolii nifH promoters

Growing community of inventors

Heidelberg, Germany

Peter Schofield