Novo Nordisk A/s

Peter Rosholm

Martin Schulein

Grethe Norskov Rasmussen

Egon Nielsen

Henrik Fredholm

Carsten Mailand Hjorth

Allan Svendsen

Kim Borch

Henrik Lund

Claus Von Der Osten

Arne Agerlin Olsen

Jack Bech Nielsen

Marianne Thellersen

Niels Munk

Svend Aage Hansen

So Slashedren Lund

Kai Chuang Heng

The present invention relates to variants of a Family 7 cellulase comprising one or more mutations of the amino acid sequence of the cellulase in which each mutation is independently a substitution of an amino acid residue with another amino acid or an insertion of at least one amino acid residues, wherein the variant has a modified H-bonding potential and hydrophobic interaction relative to the cellulase. The present invention also relates to the use of the cellulase variants in detergent compositions, especially for soil removal or color clarification or preventing backstaining; in fabric softeners; for bio-polishing of textiles; for drainage improvement of paper pulp; for deinking of old paper; for plant degradation.

Alkaline cellulases

The present invention provides variants of a parent cellulase, such as, e.g., a cellulase classified in family 45 such as a Humicola insolens 43 kD endoglucanase, comprising a cellulose binding domain (CBD), a catalytically active domain (CAD), and a region linking the CBD and CAD (the linking region), wherein one or more amino acid residues of the CBD, CAD, or linking region is deleted or substituted by one or more amino acid residues and/or one or more amino acids are added to the linking region and/or another CBD is added at the opposite end of the CAD. Such variants have improved properties as regards, e.g., alkaline activity, compatibility with detergent composition ingredients, particular soil removal, color clarification, defuzzing, depilling, harshness reduction, and sensitivity to anionic surfactants. Such variants are useful, e.g., in detergent compositions, for textile treatment, in paper pulp processing, for animal feed, and for stone-washing of jeans.

Cellulase variants

Removal of protein from natural rubber latex articles

Enzyme preparation comprising a modified enzyme

Cellulase variants and detergent compositions containing cellulase

Growing community of inventors

Pentaling Jaya, Malaysia

Peter Rosholm