Leland Stanford Junior University

Schering Corporation

The Perkin-elmer Corporation

Peter R Parham

Joseph H Phillips

Lewis L Lanier

Alan M Krensky

Carol A Clayberger

Robert B Chadwick

Leslie Johnston-Dow

Jennifer E Gumperz

Virginia M Litwin

A method for typing HLA class 1 genes. The method entails first contacting a sample DNA with first and second amplification primers, wherein the first and second primers are each at least partially located in an exonic region. Next, using the first and second primers, a target sequence is amplified by the PCR to form an amplicon of the target sequence. Finally, the amplicon is detected with a sequence-specific detection means, e.g., DNA sequencing. The invention also includes specific amplification primers, specific sequencing primers, and kits especially adapted for use with the above HLA typing method.

Methods and reagents for typing HLA class I genes

Antibodies which specifically bind to an NK cell surface antigen from a mammal, reagents related thereto, including purified proteins, specific antibodies, and nucleic acids encoding said antigen. Methods of using said reagents and diagnostic kits are also provided.

Antibodies to mammalian NK antigens and uses

Fragments from the polymorphic domains of Class I HLA antigen domains are used to modulate T-cell activity. The peptides are from the .alpha.1- or .alpha.2 domains, particularly of the HLA-A, and B antigens. The peptides may be conjugated to other compounds to be used in diagnosis and therapy. The peptides may block lysis, CTL proliferation or have other regulating effects.

Cytotoxic T-cell lymphocyte ('CTL') activity regulation by class I MHC

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Stanford, CA, United States of America

Peter R Parham