Dsm Fine Chemicals Austria Nfg Gmbh & Co. Kg

Patheon Austria Gmbh & Co. Kg

Esim Chemicals Gmbh

Peter Pöchlauer

Helmut Schwab

Anton Glieder

Wolfgang Skranc

Irma Wirth

Herbert Mayrhofer

Rudolf Neuhofer

Christoph Kratky

Ingrid Dreveny

Rodolfo Bona

Stefan Steinhofer

Rolf Marr

Martina Kotthaus

Thomas Zich

Martin Vorbach

Paul Kohls

Martin Deak

Christian Schuster

The invention pertains to a process for preparing a compound of formula (1)    and

Process for preparing pentenoate

The invention relates to a method for carrying out reactions with participation of carbocations, whereby the initial most strongly exothermic phase of the reaction is carried out at high temperature (60 to 120&#xb0; C.) and short residence time (1 to 30 seconds) in a microreactor and the subsequent less exothermic phases are carried out at optionally lower temperatures in two or more residence time units with longer residence times (1 to 30 seconds).

Stepwise execution of exothermic reactions with participation of carbocations

New genes containing a DNA sequence coding for hydroxynitrile lyase, which genes can be prepared via a primer combination based on the DNA sequence of the 5'-region of the mdl genes from theand fromand/or a primer 2 based on the 3&#x2032;-region of the DNA sequences of one of the hydroxynitrile lyase isoenzymes fromor from, subsequent amplification with a DNA polymerase using a DNA from organisms, containing genes coding for hydroxynitrile lyase, as templates and cloning, and also recombinant proteins which can be prepared in suitable host cells by heterologous expression of the DNA sequence of said HNL genes, and proteins and fusion proteins derived therefrom and use of said proteins for preparing (R)- or (S)-cyanohydrins.

Isolated protein having hydroxynitrile lyase activity

New genes containing a DNA sequence coding for hydroxynitrile lyase, which genes can be prepared via a primer combination based on the DNA sequence of the 5'-region of the mdl genes fromand fromand/or a primer 2 based on the 3&#x2032;-region of the DNA sequences of one of the hydroxynitrile lyase isoenzymes fromor from, subsequent amplification with a DNA polymerase using a DNA from organisms, containing genes coding for hydroxynitrile lyase, as templates and cloning, and also recombinant proteins which can be prepared in suitable host cells by heterologous expression of the DNA sequence of said HNL genes, and proteins and fusion proteins derived therefrom and use of said proteins for preparing (R)- or (S)-cyanohydrins.

Genes containing a DNA sequence coding for hydroxynitrile lyase, recombinant proteins derived therefrom and having hydroxynitrile lyase activity, and use thereof

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Linz, Austria

Peter Pöchlauer