City of Hope

Columbia University

Other

Leland Stanford Junior University

Peter P Lee

Samuel C Silverstein

John D Loike

Sadna Budhu

Dobrin Draganov

Tor B Stuge

The disclosure provides, inter alia, methods of detecting IL-6 signaling activity in T cells in breast cancer patients, such as breast cancer patients in remission.

IL-6 signaling and breast cancer

Described herein, inter alia, are macrocyclic lactones such as ivermectin and methods for their use in treating, for example, hyperproliferative disorders such as cancer.

Macrocyclic lactones and uses thereof as modulators of purinergic receptors

A method is provided, inter alia, for treating a subject suffering from an infectious or neoplastic disease with immunotherapy. The method includes determining the critical concentration of immune cells required to treat or eradicate an infectious or neoplastic disease in the subject using an in vitro assay of the present invention; and administering to the subject the critical concentration of immune cells determined in the assay.

Methods for treating an infectious or neoplastic disease

A method is provided for treating a subject suffering from an infectious or neoplastic disease with immunotherapy. The method comprises determining the critical concentration of immune cells required to treat or eradicate an infectious or neoplastic disease in the subject using an in vitro assay of the present invention; and administering to the subject the critical concentration of immune cells determined in the assay.

An in vitro assay is provided for determining the effect of an immune cell on a cell from an infectious or neoplastic disease. Also provided is an in vitro assay for determining the effect of an activated CD8T-cell on a sensitized melanoma cell. A method for improving the specific cytolytic activity (SCA) of an immune cell comprising contacting an immune cell with an antigen and an antigen-independent pro-inflammatory agent is provided. A method for ex vivo expansion of antigen-specific CD8T-cells with enhanced specific cytolytic activity (SCA) comprising culturing the antigen-specific CD8T-cells in a suitable culture media comprising an amino acid. An in vitro assay is provided for determining the effect of an immune cell on a cell from an infectious or neoplastic disease. A method of treating a subject suffering from an infectious or neoplastic disease with immuno therapy is described.

Assays for determining the effect of an immune cell on a cell from an infectious or neoplastic disease

Methods and compositions for producing target cell reactive lymphocytes, e.g., cytolytic T-lymphocytes, in a subject are provided. In practicing the subject methods, a lymphocyte population is contacted with an effective amount of a target cell peptide mixture of active and inactive peptides to produce lymphocytes reactive, e.g., cytolytic, for the target cell. Also provided are compositions, kits, and systems for practicing the subject methods. The subject invention finds use in a variety of different applications, including therapeutic applications.

Methods for producing target cell reactive lymphocytes

A non-caking shoe polish composition comprising in addition to a traditional base mixture of paraffin wax and turpentine oil, refined petroleum jelly, silicone oil, and optionally, camphor oil, silicone powder, and color ink. The composition may be usefully impregnated into a shoe polish cloth consisting of a section of fabric material adapted for easy and non-messy application of the composition onto leather or imitation leather materials.

Growing community of inventors

San Marino, CA, United States of America

Peter P Lee