Novartis Ag

Ciba-geigy Corporation

Bayer Technology Services Gmbh

Peter Oroszlan

Michael Pawlak

Gert Ludwig Duveneck

Gerolf Kraus

Carlo S Effenhauser

Eginhard Schick

Alfredo Emilio Bruno-Raimondi

Andreas Helg

Aran Paulus

Kees Ensing

A device has a planar optical waveguide, including a transparent carrier ( ) and a wave-guiding layer ( ), wherein the waveguide at least has a diffractive element ( ) for coupling excitation radiation into the wave-guiding layer. Located on the wave-guiding layer is a further, tightly sealing layer ( ) made from a material which, at least at the support surface, is transparent both to the excitation radiation and to the evanescently excited radiation at least to the penetration depth of the evanescent field, and which, for an analysis sample, has, at least in a partial region of the guided excitation radiation, a cavity ( ) which is open to the upper side or a cavity ( ) which is closed to the upper side and connected by means of an inlet channel ( ) and an outlet channel ( ). The depth of the cavity corresponds at least to the penetration depth of the evanescent field, and the diffractive element ( ) is fully covered by the material of the layer ( ) at least in the coupling-in region of the excitation radiation.

Measuring device and the use thereof

A device comprising a planar optical waveguide, consisting of a transparent carrier (,) and a wave-guiding layer (,), wherein the waveguide at least has a diffractive element (,) for coupling excitation radiation into the wave-guiding layer, and there is located on the wave-guiding layer a further, tightly sealing layer (,) made from a material which, at least at the support surface, is transparent both to the excitation radiation and to the evanescently excited radiation at least to the penetration depth of the evanescent field, and which, for an analysis sample, has, at least in a partial region of the guided excitation radiation, a cavity (,) which is open to the upper side or a cavity (,) which is closed to the upper side and connected by means of an inflow channel (,) and an outflow channel (,), the depth of the cavity corresponding at least to the penetration depth of the evanescent field, and wherein the diffractive element (,) is fully covered by the material of the layer (,) at least in the coupling-in region of the excitation radiation.

Measuring device and its use

A device for combined bioaffinity assay and electrophoretic separation is provided, which comprises a capillary system having two stages, a first stage in which bioaffinity interactions of analyte molecules and molecular recognition elements are performed, and a second stage, in which electrophoretic separation of the analyte molecules and subsequent detection of the separated species is accomplished. Within the first capillary stage the molecular recognition elements are attached and immobilized to the inside capillary wall, for example, by adsorption or by covalent binding to the capillary material. The method for combined bioaffinity assay and electrophoretic separation comprises flowing an analyte through a capillary system having two stages. In a first capillary stage the analyte molecules are captured by respective molecular recognition elements present in that stage. More particularly the analyte molecules are captured by molecular recognition elements which are attached and immobilized to the inside wall of that capillary stage, for example, by adsorption or by covalent binding to the capillary material. After a predetermined time the analyte-molecules are dissociated from the molecular recognition elements. Subsequently the analyte-molecules are separated in a second stage of the capillary system by capillary electrophoresis and finally the separated species are detected at the terminal part of the capillary system.

Device and method for combined bioaffinity assay and electrophoretic

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Basel, Switzerland

Peter Oroszlan