Thermo Fisher Scientific Baltics Uab

Oulun Yliopisto

Iep Gmbh

Biosilta Oy

Enpresso Gmbh

Peter Neubauer

Juozas Siurkus

Antti Vasala

Antje Gupta

Antje Neubauer

Klaus Breese

Johanna Panula-Perala

Gert Bange

Russell Golson

Antii Vasala

A method for producing from host cells a heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state. The method cultured the host cells in a culture medium comprising a reducing agent; and recovered the heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state from the host cells or from the culture medium, where the host cells comprise a nucleic acid encoding the heterologous protein or polypeptide, and where the reducing agent is capable of permeating the plasma membrane of the host cells.

Protein production

In a method for controlling the growth of microbes grown in a medium to high cell-densities by the fed-batch technology, a two-phase system, having a liquid phase (cultivation medium) and a solid or gel phase, the solid or gel phase provides a source of substrate-delivering polymer which is turned by an enzyme in a controlled way into a growth-limiting substrate or pH adjusting agent. Also described is a method for restricting the synthesis of growth-limiting metabolites and preventing oxygen-depletion in microbial cell cultures.

Method for controlling the growth of cell culture

The present invention is generally in the field of continuous and high-cell-density cultivation in laboratory- or large-scale liquid shaken cultures. More particularly it relates to a method of enzyme-based fed-batch (EnBase) for liquid microbial prokaryotic or eukaryotic cell cultivation having the possibility to manipulate the growth rate of the cultured organisms by a controlled enzymatic release of the growth-limiting substrate-monomer from substrate-polymers or substrate-oligomers.

Enzyme-based fed-batch technique in liquid cultures

The invention relates to an enzymatic method for the enantioselective reduction of organic keto compounds to the corresponding chiral hydroxy compounds, an alcohol dehydrogenase fromminor and a method for the enantioselective production of (S)-hydroxy compounds from a racemate.

Enzymic method for the enantioselective reduction of keto compounds

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Berlin, Germany

Peter Neubauer