Gist-brocades N.v.

Dsm N.v.

Gist-brocades B.v.

Peter Michael Andreoli

Leo J Mulleners

Johan Pieter Sanders

Yvonne J Vos

Jan H Van Ee

Johannes Abel Van Den Berg

Jacob B Visser

Abraham Harder

Farrokh Farin

Maria M Cox

Janna Gardina Bakhuis

Yves Coutel

Peter Van Der Veen

Michel Johannes Flipphi

Margaretha Van Heuvel

Leendert Hendrik De Graaff

Johannes A Van Den Berg

Suzanne Wohlfarth-Rippel

Leendert Hendrick De Graaff

Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected. Efficient transfer of the vehicle containing the gene of interest is achieved, with the resulting industrial strain transformants being effective, stable producers of the desired polypeptide product.

Transformed industrial bacillus strains and methods for making and using

The present invention provides the cloning and expression of purified and isolated DNA molecules, obtainable from fungi, which encode enzymes having arabinan-degrading activity. The present invention also provides DNA constructs containing these DNA molecules and methods for their enhanced expression in selected microbial host cells. The present invention further provides methods for the use of the thus-produced arabinan-degrading enzymes.

Cloning and expression of DNA molecules incoding arabinan-degrading

Cloning and expression of DNA molecules encoding arabinan-degrading

Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected. Efficient transfer of the vehicle containing the gene of interest is achieved with the resulting industrial strain transformants being effective, stable producers of the desired polypeptide product.

Novel microbial host strains are provided which are transformed by a vector molecule comprising a DNA fragment encoding a lipolytic enzyme and a marker for selection, capable of producing active lipase. Said DNA fragment is preferably derived from a Pseudomonas species.

Compositions and methods for making lipolytic enzymes

Molecular cloning and expression of gene encoding lipolytic enzyme

Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected Efficient transfer of the vehicle containing the gene of interest is achieved, with the resulting industrial strain transformants being effective, stable producers of the desired polypeptide product.

Molecular cloning and expression in industrial Bacillus species

Growing community of inventors

Belgium, Netherlands

Peter Michael Andreoli