Phylos, Inc.

Compound Therapeutics, Inc.

Bristol-myers Squibb Compnay

Arqule, Inc.

Adnexus Therapeutics, Inc.

Peter Lohse

Markus Kurz

Michael McPherson

Mark Anthony Ashwell

Robert G Kuimelis

Manish Tandon

Yanbin Liu

Syed M Ali

Belew Mekonnen

Jifeng Liu

Robert Selliah

Woj Wrona

Valery Antonenko

In general, the present invention relates to compounds capable of inhibiting p38, methods for inhibiting p38 in vivo or in vitro, and methods for treating conditions associated with p38 activity or cytokine activity.

Inhibitors of P38 and methods of using the same

Described herein are methods and reagents for the ligation of a peptide acceptor to an RNA, as well as the RNA-peptide acceptor products.

Peptide acceptor ligation methods

Disclosed herein are molecules that include a deoxyribonucleic acid (DNA) covalently bonded to a protein and uses thereof.

DNA-protein fusions and uses thereof

Disclosed herein are in vitro assays for the identification of interactions between proteins or other molecules, the identification of transcriptional activator proteins, and the detection of compounds that inhibit protein/protein or protein/compound interactions. Also disclosed herein are in vitro assays for the selection of interacting proteins and transcriptional activator proteins out of libraries.

In vitro protein interaction detection systems

In general, the invention features proteins having covalently bonded C-terminal puromycin tags and methods for their production.

C-terminal protein tagging

Disclosed herein is a method for generating a 5&prime;-nucleic acid-protein conjugate, the method involving: (a) providing a nucleic acid which carries a reactive group at its 5&prime; end; (b) providing a non-derivatized protein; and (c) contacting the nucleic acid and the protein under conditions which allow the reactive group to react with the N-terminus of the protein, thereby forming a 5&prime;-nucleic acid-protein conjugate. Also disclosed herein are 5&prime;-nucleic acid-protein conjugates and methods for their use.

Methods for producing 5&prime;-nucleic acid-protein conjugates

Disclosed herein is a method for detecting a compound-protein interaction, involving: (a) providing a compound library in which each member of the compound library is immobilized on a solid support; (b) contacting each member of the immobilized compound library in a single reaction chamber with each member of a protein-nucleic acid fusion library under conditions which allow the formation of compound-fusion complexes; (c) isolating the immobilized compound-fusion complexes; and (d) detecting a compound-fusion complex as an indication that the protein of the fusion interacts with the compound. In preferred embodiments, the protein is identified by reading the nucleic acid portion of the fusion, and the compound is identified by reading a detectable tag bound to either the compound or the solid support.

Growing community of inventors

Weston, MA, United States of America

Peter Lohse