Sru Biosystems, Inc.

Nexcelom Bioscience LLC

X-body, Inc.

University of California

Revvity Health Sciences, Inc.

Srll Biosystems, Inc.

Peter Li

Brian T Cunningham

Bo Lin

Jean Qiu

Homer Pien

Jane Pepper

Timothy Smith

Lance Gavin Laing

William C Karl

Frank DiFilippi

Leo L Chan

Gangadhar Jogikalmath

Lara Madison

John Gerstenmaier

Christine C Genick

Derek Puff

Todd Sobolewski

Alex Borsody

Cheryl Baird

Stephen C Schulz

Brant Binder

Kevin Flanagan

Alnoor Pirani

Constance J Chang-Hasnain

Justin R Hascup

Carlos Mateus

Frank DeFilippi

Nicholas N Shaw

Jordan Bell

The invention provides novel sample chambers, units and multi-well plates, and systems and methods thereof, for built-in measurement assurance of cell counting methods and calibrated and/or quality-assured measurement and analysis of diverse types of biological cells, e.g., cell count, cell size, cell concentration, cell sub-population, cell morphology, cell viability, etc.

Systems and methods for cell count measurements

The invention provides novel sample chambers, sample analysis units, multi-well plates, and imaging and analytical systems as well as methods for accurate, efficient and high-throughput imaging, measurement and analysis of diverse types of biological cells to obtain information such as cell count, cell size, cell concentration, cell sub-population, cell morphology, cell viability, etc.

System and method for cell imaging, analysis and measurement

The invention generally relates to analytical and monitoring systems useful for analyzing and measuring cells and biological samples. More particularly, the invention provides systems and methods for internal calibration and focus reference for cytometry imaging.

Internal focus reference beads for imaging cytometry

The present invention generally relates to systems and methods for counting biomolecules or cells. In certain embodiments, the invention provides a cell counting or biomolecule counting system including: a covered chamber having a known height and configured to hold a suspension of biomolecules or cells in a sample; at least one fluorescent light source connected to at least one fluorescent light beam narrowing device; a bright-field light source connected to a bright-field light beam narrowing device; a microscope objective; a detection device; a fluorescent filter assembly to allow only excitation light to illuminate the sample and allow only emission light from the sample to be imaged by the detection device; and a movable light shutter to block bright-field light during fluorescent detection.

Systems and methods for counting cells and biomolecules

The invention generally relates to analytical and monitoring systems useful for analyzing and measuring cells and biological samples. More particularly, the invention relates to systems and methods for imaging, measuring, counting, analyzing, and monitoring microscopic particles such as cells and biological molecules in solution samples.

Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.

Optical detection of label-free biomolecular interactions using microreplicated plastic

The invention provides methods of detecting a change in cell growth patterns, methods of screening many different antibodies in one receptacle, and methods of detecting specific binding of an antibody to a protein or cell, wherein the antibody is in a mixture of many different antibodies.

Methods for screening cells and antibodies

Performing high-resolution determination of the relative shift of the spectral properties of a biosensor. The shift in the resonance peak of the biosensor is indicative of the amount of material bound to the surface of the biosensor. A preferred biosensor is a Guided Mode Resonant Filter Biosensor (GMRFB). In one aspect of the invention, curve fitting is used to determine the relative location of the spectrum of the unexposed biosensor with respect to those spectra that are altered (e.g., shifted) by the presence of materials bound to the surface of the biosensor. In an alternative embodiment, the cross correlation function is used to detect spectral peak offsets between a reference spectrum and a spectrum measured from an exposed biosensor. In yet another alternative, maximal likelihood estimation techniques are used to determine the spectral shift or offs.

Method and apparatus for biosensor spectral shift detection

Label-free high-throughput optical technique for detecting biomolecular interactions

A grating-based sensor is disclosed that has a grating structure constructed and designed for both evanescent resonance (ER) fluorescence detection and label-free detection applications. Some embodiments are disclosed which are optimized for ER detection in an air mode, in which the sample is dry. Other embodiments are optimized for ER detection in liquid mode, in which the sample is suspended in liquid medium such as water. One and two-dimensional gratings are also disclosed, including gratings characterized by unit cells with central posts, central holes, and two-level, two-dimensional gratings. A readout system for such sensors is also disclosed. One embodiment includes a first light source optimized for collecting label-free detection data, a second light source optimized for collecting ER fluorescence amplification data, and at least one detector. In one embodiment, the detector is an imaging system and includes a CCD camera for collecting both ER and label-free data. In other embodiments, the at least one detector takes the form of a spectrometer for collection of label-free data and a photomultiplier for collecting ER data. In other embodiments, a single light source such as a tunable laser or broad band light source is used.

Grating-based sensor combining label-free binding detection and fluorescence amplification and readout system for sensor

Optical detection of label-free biomolecular interactions using microreplicated plastic sensor elements

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Andover, MA, United States of America

Peter Li