Cambridge Research Instrumentation, Inc.

Peter L Domenicali

Clifford C Hoyt

Peter J Miller

Scott Determan

Disclosed are methods and systems for: (i) sequentially illuminating a specimen with different spatial distributions of light, wherein each illumination causes an object embedded in the specimen to emit radiation in response to the light; (ii) for each different spatial distribution of illumination light, imaging the radiation emitted from the specimen from each of multiple sides of the specimen; and (iii) determining information about the object in the specimen based on the imaged radiation from each of the multiple sides for each of the different spatial distributions of illumination light.

Systems and methods for in-vivo optical imaging and measurement

Disclosed herein are multispectral detection methods and systems that can be used with image-forming optics configured to form an image of a sample. The systems include: (a) an imaging detector; (b) relay optics that include multiple optical elements, the relay optics positioned to relay the image formed by the image-forming optics to the imaging detector; (c) an actuator coupled to one of the optical elements in the relay optics and configured to adjust a position of the coupled optical element; and (d) control electronics configured to cause the actuator to adjust the position of the coupled optical element in the relay optics in response to a wavelength band selection by a wavelength selection element positioned to select one wavelength band for the image from among two or more wavelength bands within an overall wavelength range.

Spectral imaging system with dynamic optical correction

The invention provides for surface mapping of in-vivo imaging subjects using a single camera and an illuminator that projects a plurality of targets such as spots on the subject. By limiting the depth-of-field of the camera lens, or of the illuminator optics, or both, a spatial plane is defined in which the spots are most sharply in focus. Controlled displacement of this plane relative to the subject is achieved through movement of the mechanical stage on which a subject is placed; or through movement of the best-focus plane by adjustment of the camera, lens, or illuminator optics. Images are taken at several relative positions of the best-focus plane and the subject, and the height of individual points on the subject is determined through analysis of focus, given the known displacements. A mesh or other surface can be constructed from individual point locations, to provide a surface map of the subject. Accuracy of 0.5 mm can be readily attained for mice and similarly sized subjects.

Surface measurement of in-vivo subjects using spot projector

The invention provides for surface mapping of in-vivo imaging subjects using a single camera and a moveable stage on which a subject animal for in-vivo imaging is placed. Images are taken and the stage is moved by known amounts along the optical axis, and the heights of individual features on the subject are determined through analysis of focus, given the known stage displacement. Alternatively, height of sub-regions of the subject are determined through analysis of focus. A mesh or other surface can be constructed from individual features, to provide a surface map of the subject. Accuracy of 0.5 mm or better can be attained for mice and similarly sized subjects.

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Lynnfield, MA, United States of America

Peter L Domenicali