Pall Corporation

Other

Peter Konstantin

Yujing Yang

Chung-Jen Hou

Xiaosong Wu

Jayesh Dharia

Joel Shertok

Joe L Kinsey, Jr

Michael Ishee

Charles Gelman

Wilfrid Klaus Wixwat

Joel T Shertok

The present invention provides a positively charged microporous membrane having a protein binding capacity of about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.

Positively charged membrane

The present invention provides a hydrophilic charged microporous membrane comprising a porous hydrophobic substrate and a coating comprising a charge-providing agent. The present invention further provides a hydrophilic charged membrane comprising a porous hydrophobic substrate and a hydrophilic charge-providing agent distributed within the substrate. The present invention further provides a filter as well as a filter device comprising the inventive hydrophilic charged membrane. The present invention further provides a process for treating a fluid containing bacterial contaminants such as endotoxins comprising contacting the membrane with the fluid to provide a bacterial contaminant depleted fluid.

Charged membrane

The present invention provides, in certain embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating having fixed negative charges. The crosslinked coating can be prepared, e.g., from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- and/or N-(alkoxymethyl)-acrylamide, a hydrophilic unsaturated monomer, and an initiator. The present invention further provides, in some embodiments, a negatively charged microporous membrane comprising a porous substrate and a crosslinked coating prepared from a polymerized composition comprising an unsaturated monomer having an anionic group, an N-(hydroxymethyl)- or N-(alkoxymethyl)-acrylamide, a polysaccharide, and an initiator. The membranes of the present invention are suitable for use in ion exchange chromatography, for example, in the separation and purification of positively charged species such as proteins.

Negatively charged membrane

Filter device and filter element comprising at least one negatively charged membrane

The present invention provides a positively charged microporous membrane having a protein binding capacity about 25 mg/ml or greater comprising a hydrophilic porous substrate and a crosslinked coating that provides a fixed positive charge to the membrane. The present invention further provides a positively charged microporous membrane comprising a porous substrate and a crosslinked coating comprising pendant cationic groups. The membranes of the present invention find use in a variety of applications including ion-exchange chromatography, macromolecular transfer, as well as detection, filtration and purification of biomolecules such as proteins, nucleic acids, endotoxins, and the like.

A hydrophilic porous membrane is provided which comprises a network of a non-crystalline hydrophobic polymer and an in situ crosslinked hydrophilic acrylate polymer. A method of preparing such a hydrophilic porous membrane also is provided, wherein a reaction solution of a non-crystalline hydrophobic polymer, a crosslinkable polyfunctional acrylate, a polymerization initiator, and, optionally, a monofunctional monomer, is polymerized in situ and cast to provide a hydrophilic porous membrane.

Hydrophilic membrane

A hydrophilic porous membrane is provided which comprises a network of a non-crystalline hydrophobic polymer and a hydrophilic acrylate polymer. A method of preparing such a hydrophilic porous membrane also is provided, wherein a reaction solution of a non-crystalline hydrophobic polymer, a crosslinkable diacrylate, a polymerization initiator, and, optionally, a hydrophilic monoacrylate, is polymerized and/or crosslinked in situ and cast to provide a hydrophilic porous membrane.

The present invention provides a membrane comprising (a) polymer solids comprising 60 wt. % or more of a fully synthetic organic polymer and optionally about 40 wt. % or less of a polymer of natural origin and (b) a cellulose compound which allows for the detection of a biological molecule of interest, wherein the cellulose compound is uniformly distributed throughout the surface of the membrane. The present invention also provides methods of preparing and using such membranes.

Growing community of inventors

Boulder, CO, United States of America

Peter Konstantin