Becton Dickinson and Company Limited

Peter Johnson

Lingjie Wei

Keegan Owsley

David A Roberts

Peter Mage

Wilson On

Aspects of the present disclosure include methods for determining baseline noise of a photodetector (e.g., in a light detection system of a particle analyzer). Methods according to certain embodiments include irradiating a sample having particles in a flow stream, detecting light with the photodetector from the irradiated flow stream, generating data signals from the detected light and calculating a moving average mean squared error of the generated data signals to determine the baseline of the photodetector. Systems (e.g., particle analyzers) having a light source and a light detection system that includes a photodetector for practicing the subject methods are also described. Integrated circuits and non-transitory computer readable storage medium are also provided.

Methods for continuous measurement of baseline noise in a flow cytometer and systems for same

Droplet sorting modules for sorting droplets of a flow stream are described. Droplet sorting modules according to certain embodiments include a plurality of droplet sort decision units and a processor with memory operably coupled to the processor, where the memory includes instructions stored thereon, which when executed by the processor, cause the processor to determine an optimal droplet sort decision unit from the plurality of sort decision units for sorting each droplet of the flow stream. Particle (e.g., cells) sorting systems and methods for sorting droplets of a flow stream are also described. Kits having one or more of the subject droplet sorting modules are also provided.

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Santa Cruz, CA, United States of America

Peter Johnson