Ortho Diagnostics, Inc.

Ortho Pharmaceutical Corporation

Ortho Diagnostic Systems Inc.

Peter J Natale

Igino Lombardo

Ernest D Carmagnola

W Peter Hansen

Robert A Hoffman

Stefan J Kirchanski

A R Azad

Scooter

Reagents and methods for intracellular staining of anionic materials as provided. The inherent staining characteristics of a fluorescent molecule may be suitably altered by covalently linking a plurality of said molecules to a nonimmunoglobulin derived cationic polymer having the desired staining specificity. By careful selection of the net charge and size of the dye polymer complex, diffusion through the cellular membrane can be enhanced and an enhanced fluorescent signal obtained by localization of a plurality of fluorescent molecules at the site of interest.

Methods and reagents for staining intracellular components

Latex material disposed in a solvent is injected into a sheath fluid into which the solvent is soluble, but the latex is not. The solvent being jetted from a vibrating nozzle forms droplets within the flowing sheath, which is then delivered onto an inclined hydrophobic surface reposing at a low angle to the jet. As the droplet containing stream strikes and flows down the surface, its velocity is taken up as the phase separation process for forming microparticles begins.

Methods for promoting the formation of microparticles

A core/sheath vibrating system is utilized to produce core droplets within the sheath, which in turn form into microparticles through phase separation. The flow momentum is first arrested by means of an inclined surface, and the combination is thence deposited, in a continuous, helical channel extending from one end to the other of the inside of a hollow, rotating drum. Rotation of the drum moves the forming microspheres through the channel. At the exit end of the drum, the sheath fluid which carries suspended microspheres, may be filtered, and the microspheres collected and utilized.

Reaction apparatus for the formation of microspheres or microcapsules

Apparatus for promoting the formation of microparticles

Methods and compositions for quantitating reticulocytes and platelets by fluorescence in a flow cytometer. The composition comprises an aqueous solution of the dye acridine orange, citrate ion, and para-formaldehyde at a pH of about 7.4 and an isotonic osmolality. The concentrations of the various ingredients are selected to maximized dye uptake by the reticulocytes and platelets to allow for the first time the fluorescent quantitation of these cells in whole blood using a flow cytometer.

Method and reagents for quantitative determination of reticulocytes and

Fluorescence volume exclusion signals are utilized to evaluate cell volume. Cells are suspended in a medium which is furnished with a fluorescent dye which neither penetrates nor adheres to the cells. Cells are analyzed by an optical flow cytometer, with illumination which causes the dye medium to fluoresce. Passage of a cell through the sensing zone therefore reduces the amount of fluorescent medium being illuminated, and proportionally reduces the fluorescence output signal. Fluorescence volume exclusion pulse height and area are related to cell volume.

Growing community of inventors

Canton, MA, United States of America

Peter J Natale