Berkeley Lights, Inc.

Bruker Cellular Analysis, Inc.

Bruker Cellular Analysic, Inc.

Peter J Beemiller

Xiaohua Wang

Yelena Bronevetsky

Aathavan Karunakaran

Volker L S Kurz

Gregory G Lavieu

Kristin G Beaumont

Alexander J Mastroianni

Randall D Lowe, Jr

Kevin T Chapman

Natalie C Marks

Shao Ning Pei

Guido K Stadler

Thomas M Vetterli

Hansohl E Kim

Kevin D Loutherback

Vincent Haw Tien Pai

Annamaria Mocciaro

Jason M Mcewen

Amanda L Goodsell

John A Tenney

Duane Smith

Volker L Kurz

Jr Randall D Lowe

Andrew W Mcfarland

Methods are provided for the assay of secreted biomolecules using automated detection and characterization of micro-objects in a microfluidic device. The biomolecules can be secreted by cells, particularly immunological cells, such as T cells. The biomolecules being assayed can include cytokines, growth factors, and the like. Methods are also provided for assaying the cytotoxicity of a cell with respect to another, target cell. Also provided are kits and non-transitory computer-readable media in which programs are stored for causing a system comprising a computer to perform automated methods for detecting secreted biomolecules and/or cytotoxicity in a microfluidic device.

Methods for assaying biological cells in a microfluidic device

In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces of wells within a well plate to activate lymphocytes, including but not limited to T lymphocytes, in a controllable and reproducible manner.

Functionalized well plate, methods of preparation and use thereof

Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be configured to have a critical size (D) that separates activated T lymphocytes from na&#xef;ve T lymphocytes. Also provided are microfluidic devices having an array of posts configured to separate activated T lymphocytes from na&#xef;ve T lymphocytes, compositions enriched for T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating subjects suffering from a pathogenic disorder or cancer by administering such compositions.

Sorting of T lymphocytes in a microfluidic device

In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.

In situ-generated microfluidic assay structures, related kits, and methods of use thereof

Growing community of inventors

Emeryville, CA, United States of America

Peter J Beemiller