Genentech, Inc.

University of California

Other

Peter H Seeburg

Anthony J Mason

Howard M Goodman

John Shine

Herman A De Boer

Herbert L Heyneker

Herbert L Heynerer

John D Baxter

Karoly Nikolics

Deborah L Segaloff

Keith C McFarland

John P Adelman

James L Roberts

The invention relates to the purification, and cloning of receptors for the luteinizing hormone, choriogonadotropin, follicle stimulating hormone, and thyroid stimulating hormone. The invention additionally concerns the uses for such molecules in the diagnosis and therapy of human conditions.

Luteinizing hormone/choriogonadotropin (LH/CG) receptor

DNA encoding the prepro inhibin .alpha. and .beta. chains has been isolated. This DNA is ligated into expression vectors and used to transform host cells for the preparation of inhibin or activin. Also provided are prohormone domains and other inhibin .alpha. or .beta. chain derivatives having therapeutic or diagnostic interest. The compositions provided herein are useful in the manipulation of fertility in animals.

Nucleic acid encoding the mature .beta..sub.B chain of inhibin and

Nucleic acid encoding the mature .beta..sub.A  chain of inhibin and

Nucleic acid encoding the mature .alpha. chain of inhibin and method for

High-level expression of bovine growth hormone is achieved using DNA encoding bovine growth hormone which, when transcribed, produces messenger RNA (mRNA) that at its 5' end is substantially free of secondary structure that is capable of interfering with translation of the mRNA.

DNA for expression of bovine growth hormone

Nucleic acid encoding the .alpha. chain prodomains of inhibin and method

Expression levels of polypeptides in recombinant host cell culture are increased by the use of synthetic DNA sequences chosen so that the mRNA transcribed therefrom is free of secondary structure sufficient to interfere with the efficiency of mRNA translation.

Methods and products for facile microbial expression of DNA sequences

Microbial expression of an exogenous polypeptide may be inefficient if the messenger RNA has secondary structure (due to complementarity of spaced regions of the molecule) which impedes interaction with a ribosome. The occurrence of such structure can be predicted. The degeneracy of the genetic code gives freedom to alter the nucleic acid composition of the CDNA to eliminate harmful secondary structure of the MRNA. For example, bovine growth hormone (BGH) is poorly expressed by E. coli transformed with a recombinant plasmid containing the natural BGH gene. The corresponding MRNA has complementary regions at 46-51 and 73-78. Therefore a plasmid PBGH 33-4 was constructed containing a BGH gene whose upstream portion was synthesized (making use of plasmid PHGH 207-1 which contains an efficiently expressible gene for the closely related human growth hormone) so as not to cause the troublesome complementarity. E. coli transformed therewith produced BGH with-a yield of better than 10.sup.5 copies per cell.

Method for expression of bovine growth hormone

DNA encoding the prepro inhibin .alpha. and .beta. chains has been isolated. This DNA is ligated into expression vectors and used to transform host cells for the preparation of inhibin or activin. Also provided are prohormone domains and other inhibin .alpha. and .beta. chain derivatives having therapeutic or diagnostic interest. The compositions provided herein are useful in the manipulation of fertility in animals.

Nucleic acid encoding the ba chain prodomains of inhibin and method for

Nucleic acid encoding .beta. chain prodomains of inhibin and method for

DNA encoding a novel polypeptide hormone, designated CTP, has been identified in the human genome. CTP is expressed as a fusion protein (proLHRH) with luteinizing hormone releasing hormone (LHRH), and is post-translationally processed to CTP and LHRH. CTP, its derivatives, and its fusions, including proLHRH, are useful in therapy and diagnostics.

Prepro-LHRH C-terminal peptide DNA

Novel amino terminal methionyl embodiments of bovine growth hormone are prepared in recombinant cell culture. Amino terminal methionyl derivatives of bovine growth hormone are particularly useful in enhancing milk production by dairy cattle.

Terminal methionyl bovine growth hormone and its use

Compositions of hormonally active human and porcine inhibin containing

A novel non-allelic human growth hormone variant encoding gene is isolated using recombinant DNA techniques. The human growth hormone variant gene or a human growth hormone variant gene fused to a presequence may be inserted into cloning or expression vehicles which in turn may be used to transform cells in culture.

DNA vectors encoding a novel human growth hormone-variant protein

A novel non-allelic human growth hormone variant protein is disclosed. The structure of the protein is determined and the protein product is produced by recombinant DNA methods.

Growing community of inventors

San Francisco, CA, United States of America

Peter H Seeburg