Helmholtz-zentrum Fur Infektionsforschung Gmbh

Helmholtz-zentrum Fuer Infektionsforschung Gmbh

Peter Golyshin

Manuel Ferrer

Kenneth Timmis

Wolf-Rainer Abraham

Kenneth N Timmis

Julia Sabirova

Heinrich Lunsdorf

Olga Golyshina

Tatjana Chernikova

Michail Yakimov

Heinrich Lünsdorf

Julia Lab Of Industrial Microbiology And Biocatalysis Sabirova

The present invention is in the field of biosynthesis of polyhydroxyalkanoates (PHA). The invention relates to a genetically engineered microorganism having at least one gene involved in the metabolism, preferably in the production, of polyhydroxyalkanoates (PHA). This microorganism is useful in commercial production of polyhydroxyalkanoates. The present invention further relates to a method for the production of polyhydroxyalkanoates (PHA).

Extracellular polyhydroxyalkanoates produced by genetically engineered microorganisms

The present invention relates to enzymes having catalytic activity at a pH below 5.0. The present invention provides hydrolyzing enzymes obtainable from archaeobacteria, in detail to hydrolytic enzymes obtainable from the archaeobacterium. In general, the present invention provides enzymes which are active and stable at acidic pH values, especially at pH values from 1 to 4, especially in the range of pH 2 to 3, obtainable from, especially to an esterase, glycosidases and a DNA ligase. In addition to stability and activity at low pH values, the enzymes according to the present invention are all dependent on Fefor their catalytic activity.

Acidophilic enzymes

The invention relates to the growth temperature of organisms, especially plants and microorganisms and the manipulation of the tolerable cultivation temperature. More specifically, the present invention relates to the expression of heterologous proteins in microorganisms, and especially to the heterologous expression of heat sensitive proteins in bacteria, either gram-negative or gram-positive. In a first aspect, the present invention provides a method for manipulation of cells and the resultant cells, wherein at least one gene from a psychrophilic micro organism coding for at least one chaperone or chaperonin is expressed. Such cells are selected among cultivated eukaryotic cells, i.e. animal and plant cells and entire plants, gram-negative and gram-positive bacteria, fungi and yeasts.

Transgenic organisms with lower growth temperature

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Wolfenbuttel, Germany

Peter Golyshin