Synapse B.v.

Peter Giesen

Hendrik Coenraad Hemker

Robert Wagenvoord

Suzette L Beguin

Sebastiaan Nijhuis

Raed Al-Dieri

Raed Al Dieri

Suzette Beguin

Sabastiaan Nijhuis

The invention relates to a method for in vitro determining thrombin activity in a sample wherein the sample is a blood sample and thrombin generation is measured by the steps of: &#x2014;contacting a layer of said sample with a fluorogenic substrate of thrombin, wherein said layer has a thickness within a range of 0.05 to 5 mm and a surface within a range of 10 to 500 mm2; &#x2014;allowing thrombin to generate in said sample; &#x2014;measuring the fluorescence emitted from the surface of the layer, by the fluorescent group released from the fluorogenic substrate as a result of enzymatic action of generated thrombin on said fluorogenic substrate.

Measuring thrombin activity in whole blood

A method is provided for determining in real time the course of thrombin activity in a sample of blood or plasma as it appears in and disappears from the simple which comprises adding a thrombin substrate to the sample that, per unit time, produces a detectable signal in a quantity that bears relation to the amount of thrombin present. Simultaneously, in a control sample of the same blood or plasma in which thrombin generation is not triggered, the activity of a standard preparation with invariable thrombin activity is measured. The exact molar amount of thrombin present at any moment is obtained by comparison of the activity measured in clotting blood and the simultaneously measured calibrator. The method is useful inter alia for diagnosing hyper- and hypo-coaguable states, either congenital, acquired or drug-induced in humans and animals. Also provided is a kit for use in this method.

Diagnostic test for determining the concentration of transient proteolytic activity in composite biological media

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Maastricht, Netherlands

Peter Giesen