Definiens Ag

Peter Ellenberg

Guenter Schmidt

Maria Athelogou

Ralf Schoenmeyer

Günter Schmidt

Ralf SCHÖNMEYER

A method for co-registering images of tissue slices stained with different biomarkers displays a first digital image of a first tissue slice on a graphical user interface such that an area of the first image is enclosed by a frame. Then a portion of a second image of a second tissue slice is displayed such that the area of the first image enclosed by the frame is co-registered with the displayed portion of the second image. The displayed portion of the second image has the shape of the frame. The tissue slices are both z slices of a tissue sample taken at corresponding positions in the x and y dimensions. The displayed portion of the second image is shifted in the x and y dimensions to coincide with the area of the first image that is enclosed by the frame as the user shifts the first image under the frame.

Evaluation of co-registered images of differently stained tissue slices

High-resolution digital images of adjacent slices of a tissue sample are acquired, and tiles are defined in the images. Values associated with image objects detected in each tile are calculated. The tiles in adjacent images are co-registered. A first hyperspectral image is generated using a first image, and a second hyperspectral image is generated using a second image. A first pixel of the first hyperspectral image has a first pixel value corresponding to a local value obtained using image analysis on a tile in the first image. A second pixel of the second hyperspectral image has a second pixel value corresponding to a local value calculated from a tile in the second image. A third hyperspectral image is generated by combining the first and second hyperspectral images. The third hyperspectral image is then displayed on a computer monitor using a false-color encoding generated using the first and second pixel values.

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Munich, Germany

Peter Ellenberg