Affymetrix, Inc.

Abbott Laboratories Corporation

Sequoia-turner Corporation

Peter E Lobban

Mark S Chee

Earl A Hubbell

Robert J Lipshutz

Maureen T Cronin

Edward L Sheldon

Charles Garrett Miyada

Xiaohua C Huang

Stephen P A Fodor

Donald M Besemer

MacDonald S Morris

Michael C Norris

Andrew B Carlson

Young Ran Kim

Vernon L Chupp

Roderick W Larue

John Paul Stuart

Stephen P Fodor

Macdonald S Morris

Steven V Muller

Thomas R Gingeras

Andrew Stefanski

Alfred H Sturtevant

Michael G Konicek

Xiaohua X Huang

Nila Shah

The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the biotransformation genes, such as cytochromes P450. For example, one such array comprises four probe sets. A first probe set comprises a plurality of probes, each probe comprising a segment of at least three nucleotides exactly complementary to a subsequence of a reference sequence from a biotransformation gene, the segment including at least one interrogation position complementary to a corresponding nucleotide in the reference sequence. Second, third and fourth probe sets each comprise a corresponding probe for each probe in the first probe set. The probes in the second, third and fourth probe sets are identical to a sequence comprising the corresponding probe from the first probe set or a subsequence of at least three nucleotides thereof that includes the at least one interrogation position, except that the at least one interrogation position is occupied by a different nucleotide in each of the four corresponding probes from the four probe sets.

Arrays of nucleic acid probes for analyzing biotransformation genes

The invention provides methods of comparing a target nucleic acid with a reference nucleic acid using nucleic acid arrays.

Method of comparing a target nucleic acid and a reference nucleic acid

The invention provides chips of immobilized probes, and methods employing the chips, for comparing a reference polynucleotide sequence of known sequence with a target sequence showing substantial similarity with the reference sequence, but differing in the presence of e.g., mutations.

Arrays of nucleic acid probes on biological chips

A device for transporting cartridges comprises a housing for holding a plurality of cartridges in a temperature controlled environment. A transport system is also provided and has a grasping mechanism for grasping one of the cartridges. The transport system is further used to remove the cartridge from the housing and to place the cartridge into a scanner.

Cartridge loader and methods

The present invention provides systems and methods for self-calibrated measurements, for example temperature sensing. The system includes a controller, a multiplexer of low on-resistance, at least two calibration reference resistors, and a current-to-frequency converter that performs self-calibrated temperature sensing with temperature sensing devices such as resistance temperature detectors (RTDs). In general, the system provides a self-calibrated temperature sensing by the current-to-frequency converter providing a constant voltage sequentially to at least two calibration resistors and one or more RTDs using switches of low on-resistance in the multiplexer, which is controlled by the controller. The value of one of the reference resistors provided is correlate to the resistive value of the RTD at the minimum temperature of the operating temperature range for the processing equipment to be monitored, the value of another reference resistor provided is correlate to the resistive value of the RTD at the maximum temperature of the operating temperature range for the processing equipment, and the values of the remaining reference resistors, if provided, may correlate to the resistive values of the RTD at selected intermediate temperatures within the temperature range for the processing equipment. The frequency output by the current-to-frequency converter varies directly with current variation and thus inversely with the resistance of the individual calibration resistor or RTD. The controller determines-composite resistance for the calibration resistors and RTDs inclusive of the other component connecting the calibration resistors and RTDs to the current-to-frequency converter, from the frequencies output by the current-to-frequency converter. A calculation is performed using these resistances to determine an accurate self-calibrated temperature for the RTDs and the equipment to which it is associated. Further, the present invention provides the measurement system may be constructed so as to provide self-calibrated temperature measurements of one or more locations in a processing chamber which may operate within different temperature ranges or to provide self-calibrated temperature measurements of one or more a processing chambers. The present invention may also provide even more accurate self-calibrated temperature measurements by utilizing multiple reference resistors so as to provide piecewise linear calibration within the temperature operating range of the processing equipment.

System and method for self-calibrating measurement

The invention provides methods for detecting variations in polymorphic sites and/or variations in gene copy number. The methods are particularly useful for analysis of biotransformation genes, such as cytochromes P450.

Analysis of genetic polymorphisms and gene copy number

The present invention generally provides novel apparatuses for rapidly and efficiently carrying out repeated hybridizations of a target nucleic acid to an array of nucleic acid probes. The apparatus generally includes a fluid delivery system, a fluid mixing system, a temperature control system and a process control system integrated into a single device.

Fluidics station injection needles with distal end and side ports and method of using

The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the biotransformation genes, such as cytochromes P450. For example, one such array comprises four probe sets. A first probe set comprises a plurality of probes, each probe comprising a segment of at least three nucleotides exactly complementary to a subsequence of a reference sequence from a biotransformation gene, the segment including at least one interrogation position complementary to a corresponding nucleotide in the reference sequence. Second, third and fourth probe sets each comprise a corresponding probe for each probe in the first probe set. The probes in the second, third and fourth probe sets are identical to a sequence comprising the corresponding probe from the first probe set or a subsequece of at least three nucleotides thereof that includes the at least one interrogation position, except that the at least one interrogation position is occupied by a different nucleotide in each of the four corresponding probes from the four probe sets.

Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same

Fluidics station with a mounting system and method of using

The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the CFTR gene.

Arrays of nucleic acid probes and methods of using the same for

A device for analyzing a whole blood sample is provided. The device comprises a conventionl hematology analyzer integrated with a fluorescence cyometry analyzer. A controller is provided for controlling the analyzers, obtaining and utilizing data from both and reporting a quantitative result. Methods are also provided for analyzing a whole blood sample. One such method comprises the steps of performing on a single instrument an analysis of impedance associated with the blood sample, an analysis of light scatter associated with the blood sample, and an analysis of fluorescence associated with the blood sample. Data is collected and utilized. A result is reported.

Method and apparatus for performing automated analysis

DNA chips containing arrays of oligonucleotide probes can be used to determine whether a target nucleic acid has a nucleotide sequence identical to or different from a specific reference sequence. The array of probes comprises probes exactly complementary to the reference sequence, as well as probes that differ by one or more bases from the exactly complementary probes.

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Palo Alto, CA, United States of America

Peter E Lobban