Other

Agency for Science, Technology and Research

Boehringer Ingelheim Pharma Kg

Nanyang Technological University

Boehringer Ingelheim Pharma Gmbh & Co Kg

Dröge, Peter

Peter Dröge

Barbara Enenkel

Harshyaa Makhija

Farid Ghadessy

Sabrina Peter

Jia Wei Siau

Shree Harsh Vijaya Chandra

The present invention refers to lambda integrases comprising at least one amino acid mutation at positions 43, 319 and 336 of the lambda integrase as set forth in SEQ ID NO: 1. The invention further refers to nucleic acid molecules comprising the nucleotide sequence encoding the mutant lambda integrase and to host cells containing these nucleic acid molecules. The invention also refers to methods of recombining a nucleic acid of interest into a target nucleic acid in the presence of the mutant lambda integrase and sequence specific recombination kits.

Mutants of the bacteriophage lambda integrase

The present invention provides a method of stably integrating a DNA sequence of interest into a target genomic DNA sequence of a host cell, wherein the target genomic DNA sequence comprises a nucleotide sequence that is at least 80% homologous to the sequence as set forth in SEQ ID NO:1, as well as a kit for use in said method. Also provided is a method of generating a circular DNA construct essentially consisting of a DNA sequence of interest and a nucleotide sequence that is at least 80% homologous to the sequence as set forth in SEQ ID NO:3.

Site-specific DNA recombination

The present invention relates to a method of sequence-specific recombination of DNA in eukaryotic cells, comprising the introduction of a first DNA comprising a nucleotide sequence containing at least one recombination sequence into a cell, introducing a second DNA comprising a nucleotide sequence containing at least one further recombination sequence into a cell, and performing the sequence specific recombination by a bacteriophage lambda integrase Int.

Sequence specific DNA recombination in eukaryotic cells

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Singapore, Singapore

Peter Dröge