The United States of America, As Represented by the Secretary, Department of Health and Human Services

Peter D Burbelo

Michael J Iadarola

Adriana Marques

Joseph A Kovacs

Thomas B Nutman

Doran Fink

Joseph Kubofcik

Methods for detecting antigen-specific antibodies in a biological sample are described. The disclosed methods can be used for the diagnosis of a variety of autoimmune and infectious diseases. The methods use a neodymium magnet to efficiently isolate immune complexes. The disclosed methods are rapid, highly specific and sensitive, require very small volumes of biological sample, and do not require the use of radioactivity. With these advantageous features, the disclosed methods are amendable for point-of-care testing (POCT), which is currently not available for the detection of autoantibodies associated with autoimmune disease or for the detection of many pathogen-specific antibodies.

Method and device for detecting antigen-specific antibodies in a biological fluid sample by using neodymium magnets

The invention provides compositions, methods, and kits for the diagnosis or detection of infection by a pathogen that causes Lyme disease in a subject.

Compositions and methods for screening for lyme disease

The polynucleotide encoding the antigen Wb123 from the filarial nematode, the major causative organism of lymphatic filariasis is provided, along with the polypeptide encoded by the polynucleotide. Methods for making the WM23 antigen, recombinant vectors encoding the Wb123 polynucleotide, and methods of detection of the Wb123 antigen through luciferase immunoprecipitation, ELISA and other detection systems are also provided.

Diagnostic assays and methods of use for detection of filarial infection

The invention provides compositions, methods, and kits for the diagnosis or detection of active, latent, or prior infection with human herpesvirus 8 in a subject sample.

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Washington, DC, United States of America

Peter D Burbelo