Sanofi Pasteur Biologics, LLC

Sanofi Pastuer Biologies, LLC

Collagen Matrix Technologies, Inc.

Sanofi Pasteur Inc.

Peter Ciarametaro

Richard L Fahrner

C Russell Middaugh

Dale P DeVore

Daniel C Vellom

James E Woiszwillo

Paul DeGeorge

Paul Degeorge

Russell C Middaugh

This invention relates to compositions includingtoxins and/or toxoids and corresponding methods. The compositions of the invention include one or more excipients that increase stability and/or decrease aggregation of the toxins.

Pharmaceutical compositions containingtoxoids A and B

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This invention provides pharmaceutical compositions, such as vaccines, and methods of making and using such compositions.

Stabilization of vaccines by lyophilization

Methods are provided for processing and dispersing animal tissues that involve exposing processed (e.g., decellularized) tissue to an acylating agent, wherein the ratio of acylating agent to wet tissue weight is about 0.003:1 or less. Preferably, decellularized tissue is exposed to an amount of the acylating agent of about 0.1% to about 0.3% of wet tissue weight for a time ranging from about 30 seconds to about 10 minutes. A dispersed tissue matrix is produced that has a high degree of resistance to digestion by non-collagenase proteases, such as trypsin. In order to produce sufficiently high yields of these trypsin-resistant compositions, the processed tissue is preferably cryomilled to increase its surface area prior to acylation. The dispersed acylated decellularized tissue may be in the form of an injectable composition that can be introduced into tissue of a subject.

Processing animal tissues by decellularizing, increasing surface area and acylating

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Gloucester, MA, United States of America

Peter Ciarametaro