Invitek Gmbh

Stratec Biomedical Ag

Other

Roche Diagnostics Gmbh

Essentra Pte. Ltd

Invitek Gesellschaft Für Biotechnik & Biodesign Mbh

Nl Nanomed Limited

Stratec Molecular Gmbh

Schulz, Jörg

Bendzko, Peter, Dr.

Institut Für Molekular Und

Invitek Gesellschaft Für Biotechnologie & Biodesign Mbh

Peter Bendzko

Timo Hillebrand

Hans Joos

Horst Menzler

Hans Schels

Gerd Kleinhammer

Ralf Griebel

Hermann Bauer

Hans-Jürgen Hoffmann

Ute Fink

Bernd Maciej

Hans-J&uuml;rgen Hoffman

Lars-Erik Peters

Hans-Christoph Berndt

Adam Albakov

Klaus Gulbin

Oleg Wladimirowitsch Drogobych

Alexander Viktorowitsch Kuzin

Jörg Schulz

Embodiments relate to a lysis buffer mixture that is stable in storage for isolating nucleic acids from biological, preferably diagnostic samples. The mixture is preferably associated with an extraction control. The aim of the invention is to provide an improved nucleic acid extraction system, which is cost-effective, stable and easy to use, thus fulfilling the requirements of a modern nucleic acid extraction system and containing, among other things, extraction controls. Embodiments relate to a lysis buffer mixture for isolating nucleic acids, said mixture containing non chaotropic salts, a special selection of detergents, a defined quantity of at least one nucleic acid as an extraction control, optionally lytic enzymes, optionally carrier nucleic acids and optionally other additives.

Stable lysis buffer mixture for extracting nucleic acids

Disclosed is a smokeless cigarette system comprising a reusable cigarette tube and a nicotine stick which can be discarded after use. The nicotine stick comprises a depot filter charged with a nanobead solution and nicotine and flavor substances attached thereto or incorporated therein, and a mouthpiece filter, which are joined together and produced at the factory stage. The disclosed smokeless cigarette is particularly suited for use in non-smoking areas to prevent stress caused by nicotine withdrawal and as a cigarette substitute, but can also be used in different types of cigarettes with varied dosage strengths to ensure gentle nicotine withdrawal and smoke disintoxication. Neither the environment nor other persons are harmed of put at risk by this system by either passive smoking or the generation of odour. The system can be handled in a simple and unproblematic manner since no heat source or heating systems are required.

Smokeless cigarette and method for the production thereof

The invention comprises an apparatusand a methodfor purifying biomolecules. Magnetizable particlesand a group of magnetizable pinsare used for binding biomoleculesto the magnetizable particlesusing a binding buffer, and for transferring the magnetizable particlesforming a particle-biomolecule complex. The apparatuscomprises an incubation unitthat allows for an upscaling of a maximum volume of a sample material. Furthermore the apparatuscomprises counter magnetswhich are arranged on cavities. Solutionscomprising the biomoleculesand the magnetizable particlesare disposed in the cavities. The use of counter magnetsimproves the quality of an eluate. Furthermore the invention comprises a systemand a methodfor a diagnostically purifying of biomolecules. The methodfurther comprises a controlling of method steps and a selection of substances and reagents (for example the magnetizable particles) based on information automatically collected from, for example, the samples and/or derived from used substances and reagents. The systemfulfills requirements of medical diagnostics.

Apparatus and method for the purification of biomolecules

The invention relates to a new multiwell filtration plate for high throughput applications in nucleic acid technology, preferably in a 96-well or 384-well format, consisting of two individual parts, which are firmly and tightly connected together. The upper part of the plate is a sample holder for holding a sample to be filtered. The lower part of the plate is an outlet part with a filter insert for receiving a sample from the sample holder part and filtering the, sample through the filter insert.

Multiwell filtration plate

Formulations and method for isolating nucleic acids from optional complex starting material and subsequent complex gene analytics

Disclosed is a method to detect clinically relevant mutations of the DNA sequence of the KI-ras oncogene in stool DNA, its use and a testkit based thereon for early diagnosis of tumors, especially tumors of the pancreas and the colon. According to the invention, the method of detection is distinguished by extraction of genomic DNA from stool samples in a series of cleaning operations designed to eliminate inhibitor substances, and by base-complementary hybridization reaction by adding six different oligonucleotides with a defined complementarity to the clinically relevant mutated sequence fragments of the KI-ras gene.

Method to detect clinically relevant mutations of the DNA sequence of ki-ras oncogene, its use and a testkit for early diagnosis of tumors

Disclosed is a device for a simultaneous multiple isolation and purification of nucleic acids from biological material based on binding nucleic acid to mineral carrier particles. The device includes a square-shaped plastic case, a shaking apparatus with sample vessels, and a shielding for the motor and shaking apparatus.

Device for the simultaneous isolation of genomic DNA and high-purity

Method and apparatus for carrying out biochemical reactions in particular for cell-free polypeptide biosynthesis. A bioreactor has, within a housing, a reaction chamber containing a producing system as well as a supply chamber separated from the reaction chamber by means of a semi-permeable membrane and containing a liquid serving as supply medium for the producing system. The supply chamber is subdivided within the bioreactor into a first partial chamber and a second partial chamber which are each connected to the reaction chamber via a semi-permeable supply membrane and which are connected to each other by means of a connection conduit. During the biochemical reaction, liquid is circulated from the first partial chamber of the supply chamber through the reaction chamber and into the second partial chamber of the supply chamber in such a fashion that, during each passage of liquid out of the first partial chamber into the reaction chamber, liquid simultaneously passes from the reaction chamber into the second partial chamber and liquid flows out of the second partial chamber via the connection conduit back into the first partial chamber.

Method and device for carrying out biochemical reactions

Method for the simultaneous isolation of genomic DNA and high-purity RNA

A universal process is disclosed for extracting and purifying nucleic acids from extremely small amounts of highly contaminated various biological and other starting materials. The invention has applications in forensic medicine, medical diagnosis, molecular biology, biochemistry, genetic technology and all related fields. The process is characterized in that nucleic acid-containing materials are lysed, the lysate is incubated with a non-porous, non-structured, highly disperse, homogeneous and chemically pure SiO.sub.2 substrate, the substrate is isolated with the bound nucleic acids and washed with a buffer solution, then the nucleic acids are dissolved from the substrate by a buffer with a lower salt concentration. Lysis of the material and nucleic acid immobilization are preferably carried out in a reaction vessel. The substrate particles have a size of 7-40 nm, preferably 40 nm, and a specific surface from 50-300 g/m.sup.2, preferably 50 g/m.sup.2.

Universal process for isolating and purifying nucleic acids from

Growing community of inventors

Berlin, Germany

Peter Bendzko