Leland Stanford Junior University

Duke University

Advanced Liquid Logic, Inc.

Peter B Griffin

Vamsee K Pamula

Michael G Pollack

Richard B Fair

James D Plummer

Kailash Gopalakrishnan

Yue Liang

Dwayne J Allen

Jia Feng

Shu-Lu Chen

The present invention relates to droplet-based pyrosequencing including a method of identifying a base at a target position in a sample nucleic acid. The method includes: (a) providing a droplet microactuator including a first droplet including a sample nucleic acid immobilized on a bead; and (b) on the droplet microactuator: (i) contacting the first droplet with one or more reagent droplets to yield a second droplet, wherein the one or more reagent droplets include reagents for extending a double stranded portion of the sample nucleic acid by incorporating a nucleotide at the target position; (ii) splitting the second droplet to yield a third droplet including the bead and a fourth droplet lacking the bead; and (iii) assaying the third droplet to determine whether the nucleotide was incorporated at the target position.

Droplet-based pyrosequencing

Single-crystalline growth is realized using a liquid-phase crystallization approach involving the inhibition of defects typically associated with liquid-phase crystalline growth of lattice mismatched materials. According to one example embodiment, a semiconductor device structure includes a substantially single-crystal region. A liquid-phase material, such as Ge or a semiconductor compound, is crystallized to form the single-crystal region using an approach involving defect inhibition for the promotion of single-crystalline growth. In some instances, this defect inhibition involves the reduction and/or elimination of defects using a relatively small physical opening via which a crystalline growth front propagates. In other instances, this defect inhibition involves causing a change in crystallization front direction relative to a crystallization seed location. The relatively small physical opening and/or the change in crystalline front direction may be implemented, for example, using a material that is substantially unreactive with the liquid-phase material to contain the crystalline growth.

Crystalline-type device and approach therefor

The present invention relates to a droplet microactuator and to systems, apparatuses and methods employing the droplet microactuator for executing various protocols using droplets. The invention includes a droplet microactuator or droplet microactuator system having one or more input reservoirs loaded with reagents for conducting sequencing protocols, such as the reagents for conducting a pyrosequencing protocol. The invention also includes a droplet microactuator or droplet microactuator system, having one or more input reservoirs loaded with a sample for conducting a pyrosequencing protocol.

Negative differential resistance devices are implemented to facilitate current flow under different operating conditions. According to an example embodiment of the present invention, an NDR device is arranged for selective passage of current through relatively high tunneling efficiency regions and relatively low tunneling efficiency regions. In some applications, a gate is used to accumulate carriers to facilitate the passage of current that is predominantly one of tunneling current and generation current, respectively, by controlling the passage of current through a relatively high tunneling efficiency region and a relatively low tunneling efficiency region. In some implementations, the NDR device is arranged to mitigate leakage in a storage device using a two-terminal connection.

Negative differential resistance devices and approaches therefor

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Growing community of inventors

Woodside, CA, United States of America

Peter B Griffin