Other

University of Toronto

Peter Aldridge

Edward Hartley Sargent

Shana Olwyn Kelley

Jason Moffat

Stephane Angers

Reza Mohamadi

Sanna Masud

David Philpott

Mahla Poudineh

Barbara Mair

Shana Kelley

Edward Sargent

Microfluidic devices, kits systems and methods are provided for high throughput phenotypic separation of magnetically labelled cell samples. The devices and methods can be used for example to sort cells based on level of target marker and to sort screen cells, such as CRISPR screen cells and other screens with a large number of target cells, to isolate target cells and putative genetic modifiers.

Microfluidic devices and methods of use

Methods and devices for magnetic profiling of target particles in a flow. There are a plurality of flow rate-reducing structures in a flow chamber. Each flow rate-reducing structure is provided with a localized magnetic attractive force, the magnetic attractive force defining a capture zone in the vicinity of the flow rate-reducing structure. The size of capture zones may be variable for different locations within the device. The magnetic attractive force, in the capture zone, is sufficiently high to overcome the drag force on a given subset of the target particles to promote capture of any particles belonging to the subset of the target particles in the capture zone. Different target particles having different magnetic susceptibility are captured in different capture zones.

Device for magnetic profiling of particles in a flow

Growing community of inventors

Toronto, Canada

Peter Aldridge