Neste Oyj

Neste Oil Oyj

Neste Corporation

Perttu Koskinen

Ossi A Pastinen

Simo Laakso

Heidi Vainio

Reijo Tanner

Mika Sipponen

Ilkka Lehtomaki

Ilkka Lehtomäki

Stephan Hetzler

Ville Pihlajaniemi

Johan-Fredrik Selin

Alexander Steinbuchel

Olavi Myllymaki

Alexander Steinbüchel

Olavi Myllymäki

Miia Mujunen

Antti Larjo

Daniel Bröker

Maria Holmback

Birgit Haschenhermes

Miina Lehesto

Miia Laamanen

Raisa Vermasvuori

Shanna Sichwart

Ville Santala

Matti Karp

Tommi Aho

Virpi Kivinen

Suvi Myllyntausta

Daniel Broker

Stefan Uthoff

Ilkka LEHTOMÄKI

Olavi MYLLYMÄKI

Stefan Hetzler

Maria Holmbäck

Miina Lehestö

The present invention relates to improved methods for producing biosynthetic products in a cascade of bioreactors. In particular the present invention relates to methods and a cascade of bioreactor systems comprising at least two bioreactors and at least one concentration unit.

Methods for continuous production of products from microorganisms

The present disclosure relates to a method for obtaining sugar and lignin fractions from lignocellulosic materials and to a method for producing fermentation products using the sugars obtained from the lignocellulose. The present disclosure also relates to a method for improving the sugar yield in the enzymatic hydrolysate by introducing a pre-treatment step of hydrothermal hydrolysis with a delignification step using a cationic compound.

Method of processing lignocellulosic material using a cationic compound

The present disclosure relates to a method for obtaining sugar and lignin fractions from lignocellulosic materials and to a method for producing fermentation products using the sugars obtained from the lignocellulose. The present disclosure relates to a method for improving the sugar yield in the enzymatic hydrolysate by introducing a pre-treatment step of hydrothermal hydrolysis and an alkaline delignification step.

Method of processing lignocellulosic material using an alkaline delignification agent

The present disclosure relates to methods for producing microbial lipids. The present disclosure also relates to methods for producing microbial lipids using inhibitors obtainable from lignocellulosic materials to suppress the proliferation of unwanted microorganisms in the fermentation broth. The method can therefore reduce the risk of having contaminating microbes establish in the system and the cultivation and thus higher yields of microbial lipids may be obtained.

Method for producing single cell oil from lignocellulosic materials

This invention relates to cells and methods for producing lipids using cellulosic carbon source. More specifically the invention relates to oleaginous bacterial cells, wherein genes encoding at least one cellulolytic activity has been introduced. This invention also relates to methods for lipid production by cultivating an oleaginous bacterial strain or strains capable of expressing one or more cellulolytic activity.

Oleaginous bacterial cells and methods for producing lipids

Disclosed is an integrated process comprising first and second biotechnical processes. The first process produces biofuel and/or starting material for biofuel and uses a microorganism capable of producing enzymes. The second process produces biofuel and/or starting material for biofuel. In the integrated process, the microorganisms are cultivated and biofuel and/or starting material for biofuel and enzymes are produced. The microorganism culture, supernatant or a protein enriched fraction or a dilution of the supernatant comprising catalytically active enzyme(s) are introduced into the first and/or into the second process, or feedstock for the process(es) is treated. The invention relates also to use of the produced enzymes in biofuel production process or in other applications as an enzyme preparation or a source of enzymes. The invention relates also to use of the produced lipids and alcohols as biofuel, a component of biofuel or a starting material for biofuel production.

Integrated process for producing biofuels

The invention relates to a method for the recovery of oil from an aqueous solution containing oil and solids, as well as to the use of products obtained by this method. The method of the invention comprises at least the following steps of: Adjusting the oil- and solids-containing aqueous solution to a pH which is acidic, separating an oil- and solids-containing fraction from the acidic aqueous solution with an separation agent which is hydrophilic or assumes a hydrophilic character in response to water, recovering the oil- and solids-containing fraction separated by means of said separation agent, extracting oil away from the solids with a water-insoluble extraction agent, and recovering the oil-containing water-insoluble extraction agent.

Oil recovery method

The present invention relates to a genetically modifiedhost for lipid production. Thehost has been genetically modified to be deficient of one or more of genes A) a gene encoding fatty acyl-CoA reductase (EC1.2.1.n2), wherein said host is capable of increased production of TAGs and/or of total lipids compared to the parent host; and/or B) a gene encoding lipase (EC:3.1.1.3), a gene encoding pyruvate dehydrogenase (EC:1.2.2.2), and/or gene ACIAD 2177, or functional equivalents of any of said genes, wherein said host is capable of increased production of wax esters and/or total lipids compared to the parent host.

[object Object]

Disclosed are microorganisms of the genusor, which are genetically modified to express phosphomannose isomerase (EC5.3.1.8) and facilitated diffusion protein (EC1.3.1.74) for mannose uptake, and optionally mannofructokinase (EC2.7.1.4). The microorganisms also may be genetically modified to express xylose isomerase (EC 5.3.1.5), xylulokinase (E 2.7.1.17) and xylose proton symporter E or a high affinity ABC-transporter. The genetically modified microorganisms are capable of growing on mannose, xylose, arabinose, glucose, or galactose, or a combination thereof as the carbon source.

Microorganisms with extended substrate utilization range

The present invention relates to an integrated process, which comprises a single cell oil production process, and a pulp and/or paper industry process. The process comprises that in the single cell oil production process is used a microorganism capable of producing lipids or lipids and enzymes when cultivated on a medium comprising organic material from pulp and/or paper industry. Lipids or lipids and enzymes are produced by said microorganisms in the single cell oil production process and/or in a process connected into it. The present invention relates also to use of lipids produced in the process as biofuel or as a component of biofuel or as a starting material for biofuel production and use of enzymes produced in the lipid production process in pulp and/or paper industry or in other applications as an enzyme preparation or as a source of enzymes.

Integrated process system for single cell oil production and a pulp and/or paper industry process

Disclosed are processes for producing lipids for biofuel or lubricant andbacteria used in the processes. The processes include steps whereby bacterial cells of the germsare cultivated in a medium comprising organic waste(s) or residue(s) or mixtures thereof as carbon and/or nutrient source(s), wherein the organic waste(s) or residue(s) comprise lignocellulosic waste(s) or lignocellulosic residue(s), recovering lipids from the cells of the bacteria or from the cultivation medium, and using the recovered lipids or a fraction thereof as biofuel and/or lubricant, or as a starting material for biofuel and/or lubricant production.

Process and microorganisms for production of lipids from lignocellulosic wastes or residues

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Helsinki, Finland

Perttu Koskinen