Novozymes A/s

Novo Nordisk A/s

Per Lina Jorgensen

Martin Schulein

Kirk Matthew Schnorr

Mads Eskelund Bjornvad

Martin Tangney

Hanne Dela, Legal Representative

Christian Hansen

Steen Troels Jorgensen

Torben Vedel Borchert

Helle Outtrup

Claus Crone Fuglsang

Michael Dolberg Rasmussen

Stanislas Dusko Ehrlich

The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus, especially from a strain oforsp. The xyloglucanases exhibit high performance in conventional detergent compositions.

Family 44 xyloglucanases

The invention relates to isolated CGTases and isolated nucleic acid sequences encoding the CGTases. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the CGTases, in particular to cleaning and detergent composition comprising such CGTases.

CGTase and DNA sequence encoding same

The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genusespecially from a strain oforsp. The xyloglucanases exhibit high performance in conventional detergent compositions.

The present invention relates to a method for in vivo recombination of homologous DNA sequences. The method is a forced artificial evolution resulting in a DNA sequence encoding a polypeptide having an advantageous property.

In vivo recombination

A xyloglucanase having a relative xyloglucanase activity of at least 50% at pH 7 and either no or an insignificant cellulolytic activity is obtainable e.g. from a strain of Bacillus. A xyloglucanase comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:2 or homologues may be derived from eg,, ATCC 14580, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 88 to nucleotide 783; and a xyloglucanase comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO:4 or homologues may be derived from eg,, NCIMB 40482, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:3 from nucleotide 1 to nucleotide 1611. The xyloglucanases are useful e.g. in cleaning compositions and for treatment of cellulosic fibres.

Xyloglucan-specific alkaline xyloglucanase from bacillus

The present invention relates to methods of constructing recombinant Bacillus strains encoding a polypeptide of interest, in which method a DNA construct comprising a DNA sequence encoding the polypeptide of interest is introduced into a recipient Bacillus cell by conjugation from a donor cell.

Introduction of DNA into bacillus strains by conjugation

The present invention relates to Bacillus hosts transformed with a vector comprising a DNA sequence encoding for a cellulose binding domain (CBD) and capable of expressing said sequence, the expressed polypeptide protein consisting essentially of one or more non-catalytic domains; the cellulose binding domain having a molecular weight in the range of from 4 kD to 35 kD and being obtainable from a microorganism or from a plant, preferably from a bacterium or a fungus; the Bacillus host e.g. being one of the species Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus stearothermophilos, and Bacillus amyloliquefaciens; and a Bacillus expression vector carrying an inserted DNA sequence encoding for a cellulose binding domain; and a method for producing a cellulose binding domain polypeptide in a Bacillus host cell.

Growing community of inventors

Copenhagen, Denmark

Per Lina Jorgensen