Cytiva Sweden Ab

Ge Healthcare Bio-Sciences Ab

Per Kjellin

Stefan Sjolander

Hakan Erik Roos

Anita Larsson

Tanja Jarhede

Bharath Subrahmanya

Tomas Dalmo

Anna Lager

Hans Sjobom

The present invention relates to a method for production of an improved sensor surface for an SPR instrument, comprising forming a self assembled monolayer (SAM) on a surface and attaching ligands and protein resistant groups, preferably polyethylene glycol (PEG), directly to functional groups on said surface. The invention also relates to a sensor surface produced by these methods use thereof in SPR (surface plasmon resonance) assays or interactions.

Sensor surface for surface plasmon resonance assays

A self-adhesive layered septum is disclosed. In one example, the septum includes a first outer layer including a thermoplastic elastomer such as a styrenic block copolymer, containing styrene ethylene butylene styrene (TPE-SEBS) capable of closing at least partially an aperture formed when a needle is inserted through the layer; an adhesive second layer for adhering the septum to a mouth area of a well or container to which the septum is attachable; and a thermoplastic third layer between the first and second layers, thermobonded to the first layer and providing better adherence for the adhesive layer. The first layer includes a recess and a vent, which reduce pressure differentials in use, but together with the third layer minimize evaporation through the septum.

Septa

A method of preparing a protein-resistant reactive solid support surface is disclosed. The method comprises the steps of providing a solid support having a hydrogel coating with a plurality of binding elements, coupling a protein resistant compound to the hydrogel via a first fraction of the binding elements, and coupling at least one binding agent to the hydrogel via a second fraction of the binding elements, whereby the protein resistant compound and the at least one binding agent are co-immobilized to the hydrogel. Also the use of the reactive surface in analysis, such as immunogenicity assays, is disclosed.

Preparation and use of a reactive solid support surface

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Uppsala, Sweden

Per Kjellin