Chinese Academy of Agricultural Sciences

Feed Research Institute, Chinese Academy of Agricu

Pengjun Shi

Rui Ma

Bin Yao

Huoqing Huang

Huiying Luo

Yaru Wang

Yingguo Bai

Peilong Yang

Xia Shi

Kun Meng

Xiaoyun Su

Hong Yang

Wei Xia

Chao Shao

Heng Zhao

Provided are a fungus-sourced high-temperature neutral Family-45 cullulase as well as a coding gene and application thereof. The cullulase has optimal pH value of 5.5, and optimal temperature of 60&#xb0; C., has certain enzyme activity in alkaline condition, and has good alkali resistance, maintains about 70% enzyme activity in optimal condition after being processed at 90&#xb0; C. for 1 hour, maintains about 50% enzyme activity in optimal condition after being processed in boiling water for 1 hour, and can be well applied in c and other fields.

High-temperature neutral cellulase as well as coding gene and application thereof

Provided are a fungus-sourced high-temperature acid &#x3b2;-glucosidase as well as a coding gene, and an application thereof. The provide &#x3b2;-glucosidase has the optimal pH value of 4.5 and the optimal temperature of 75&#xb0; C., and maintains over 90% enzyme activity in the optimal condition after being processed at 60&#xb0; C. for 1 h. The re-engineering yeast strain GS115/bgl3A of the coding gene comprising the &#x3b2;-glucosidase has high fermentation level.

Fungus-sourced high-temperature acid B-glucosidase as well as coding gene and application thereof

The present invention relates to the field of genetic engineering, in particular, the present invention relates to a method for producing a phytase variant with an improved thermal stability, and a phytase variant and the use thereof. The phytase variant contains at least one proline modification, compared to the phytase fromand other mutants thereof. The phytase variants with the modification have preferably improved properties, such as the thermal stability, optimal reaction temperature, pH property, specific activity, protease resistance and performance in animal feeds.

Method for producing a phytase variant with improved thermal stability, and a phytase variant and the use thereof

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Beijing, China

Pengjun Shi