Harvard College

The Governors of the University of Alberta

Massachusetts Institute of Technology

Peng Zou

Adam Ezra Cohen

Robert Earl Campbell

Daniel Hochbaum

Daniel Jed Harrison

Samouil Leon Farhi

Yongxin Zhao

Alice Y Ting

Jeffrey Daniel Martell

Hyun-Woo Rhee

Provided herein are variants of an archaerhodopsin useful for application such as optical measurement of membrane potential. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, cells comprising the polynucleotides, and cells comprising the polypeptides; and methods of using the variants.

Optogenetic probes for measuring membrane potential

The application discloses methods, materials, and compositions for the labeling of molecules, for example, proteins, in living cells or in subcellular compartments of living cells. In particular, the application relates to proteomic analysis methods; materials and compositions and means based on direct tagging of unknown proteins with tagging enzymes (such as biotin ligase or a peroxidase) within the vicinity of a tagging substrate (such as a tyramide) within living cells, with optional targeting to specific subcellular locations by expression of genetic constructs.

In vivo proteomic mapping

Growing community of inventors

Cambridge, MA, United States of America

Peng Zou