Harvard College

California Institute of Technology

Dana-farber-cancer Institute Inc.

Boston University

Eagle Eye Research, Inc.

Peng Yin

Thomas E Schaus

Xi Chen

Ralf Jungmann

Jocelyn Yoshiko Kishi

David Y Zhang

Niles A Pierce

Mingjie Dai

Feng Xuan

Wei Sun

James Joseph Collins

Alexander A Green

William M Shih

Sungwook Woo

Jongmin Kim

Nikhil Gopalkrishnan

Sarit Agasti

Cameron Myhrvold

Diming Wei

Yonggang Ke

Chao Li

Chenxiang Lin

Maier S Avendano Amado

Sinem K Saka

Yu Albert Wang

Brian Beliveau

Steven Henry Strassmann

Jie Shen

Johannes Benedikt Woehrstein

John H Reif

Youngeun Kim

Robert Barish

Erik A Schultes

Geetha Shetty

Thomas H Labean

Dongran Han

Luvena L Ong

Juanita M Lara Gutierrez

Yu Wang

Ninning Liu

Maier Avendano

Johannes B Woehrstein

David Yu Zhang

The present disclosure provides, in some aspects, nucleic acid-based molecular tools that enable the recording of molecular structure and soluble signals as well as the programmed assembly of molecular structures.

Molecular programming tools

The present disclosure provides, in some embodiments, methods and compositions for single-molecule detection.

Conditional primer extension for single-molecule detection

Provided herein, in some embodiments, are molecular authentication methods, systems, and compositions.

Methods and compositions for molecular authentication

Provided herein are primers and primer systems having improved specificity and kinetics over existing primers, and methods of use thereof.

Compositions of toehold primer duplexes and methods of use

Provided herein, in some embodiments, are compositions and methods for proximity detection of molecular targets.

Proximity detection methods and compositions

The invention provides novel and versatile classes of riboregulators, including inter alia activating and repressing riboregulators, switches, and trigger and sink RNA, and methods of their use for detecting RNAs in a sample such as a well and in modulating protein synthesis and expression.

Compositions comprising riboregulators and methods of use thereof

Provided herein, in some aspects, are methods of imaging molecules without a microscope or other specialized equipment, referred to herein as 'microscope-free imaging (MFI).' Herein, 'molecular instruments' (e.g., DNA-based and protein-based molecules) are used, instead of microscopes, in a &#x201c;bottom-up&#x201d; approach for inspecting molecular targets.

Microscope-free imaging

The present disclosure provides, inter alia, methods and compositions (e.g., conjugates) for imaging, at high spatial resolution, targets of interest.

Quantitative DNA-based imaging and super-resolution imaging

Provided herein, in some embodiments, are methods and compositions for highly multiplexed in situ signal amplification via hairpin-mediated concatemerization.

Multiplexed signal amplification

Provided herein are molecular verification (authentication) systems, methods and compositions that use molecular (DNA/RNA) circuitry to enable specific molecularly encrypted solutions.

Molecular verification systems

The present disclosure provides, in some aspects, methods and compositions for producing nucleic acid nanostructures having little to no kinetic barriers to self-assembly.

Sequence design for efficient assembly of nucleic acid structures

The present disclosure provides, in some embodiments, methods and compositions for exponential amplification of single- and double-stranded DNA under isothermal conditions.

Programmable nucleic acid synthesis cascade

Growing community of inventors

Brookline, MA, United States of America

Peng Yin