University of California

Harvard College

The Brigham and Women's Hospital, Inc.

Peng Wu

Amit Choudhary

Elisa Franco

Basudeb Maji

Kurt Cox

Hari Subramanian

Hari K K Subramanian

Compositions and methods are provided for the inhibition of the function of RNA guided endonucleases, including the identification and use of such inhibitors.

Inhibitors of RNA guided nucleases and uses thereof

The embodiments disclosed herein utilize fluorescence polarization based preliminary screen to identify a putative set of Cas inhibitors from an initial set of candidate inhibitors. The primary screening assay is followed by secondary screening assay to validate the putative set of inhibitors selected by the preliminary screen. In some embodiments, the present disclosure includes compositions and methods are provided for the inhibition of the function of RNA guided endonucleases, including the identification and use of such inhibitors.

CRISPR protein inhibitors

Growing community of inventors

Boston, MA, United States of America

Peng Wu