Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Dow Agrosciences LLC

Agrigenetics, Inc.

Peizhong Zheng

Bo Shen

Mitchell Christian Tarczynski

William James Gordon-Kamm

Keith S Lowe

Timothy George Helentjaris

William B Allen

Gan-Yuan Zhong

Mark E Williams

Scott E Nichols

Chun Ping Li

Siva Prasad Kumpatla

Thomas W Greene

Ruihua Ren

Rebecca E Cahoon

Quinn Qun Zhu

Saverio Carl Falco

J Antoni Rafalski

Anthony John Kinney

Zhongsen Li

Hajime Sakai

Edgar Benjamin Cahoon

Guo-Hua Miao

Yiwen Fang

Nathan Joel VanOpdorp

Joan Tellefsen Odell

Bruce Allen Nagel

Jian-Ming Lee

Wei Chen

Steven Arnold Thompson

Sabine Susanne Hantke

Michele Morgante

Xiping Niu

Peter Friedemann

Dennis Fitzl

Gary L Cutter

Siva P Kumpatla

Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.

AP2 domain transcription factor ODP2 (Ovule Development Protein 2) and methods of use

AP2 domain transcription factor ODP2 (ovule development protein 2) and methods of use

A method is provided for determining the zygosity of an Rf4 gene in a corn plant. A method may include performing a first PCR assay, a second PCR assay, quantifying probes used in the first and second PCR assays, and comparing the quantified probes to determine zygosity.

Endpoint zygosity assay to detect Rf4 gene in maize

This disclosure concerns high-resolution mapping and candidate gene cloning of Rf4, a maize restorer of fertility gene that restores fertility to C-type cytoplasmic male sterility. The disclosure also relates to molecular markers that are tightly-linked to, or reside within, the Rf4 gene. In some embodiments, methods are provided whereby hybrid seeds may be produced from crosses of a male plant comprising nucleic acid molecular markers that are linked to or that reside within the Rf4 gene and a female plant carrying C-type CMS.

Maize cytoplasmic male sterility (CMS) C-type restorer RF4 gene, molecular markers and their use

Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/lineolic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.

Producing maize plants having a high oil phenotype using a marker locus genetically linked with a QTL6 region

This disclosure concerns specific naturally-occurring mutant maize cad2 genes, which altered genes contribute to the bm1 maize phenotype in particular maize lines. In some embodiments, compositions and methods are provided that utilize a nucleic acid molecule comprising a mutant cad2 gene, and/or markers linked to a mutant cad2gene.

Gene and variations associate with BM1 phenotype, molecular markers, and their use

Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods for manipulating oil and/or oleic acid content and/or oleic acid/linoleic acid ratio of a plant, for marker-assisted selection of a plant, and for marker-assisted breeding of the high oil and/or high oleic acid trait.

Compositions and polyeptides related to type 1 diacylgycerol O-acyltransferase (DGAT)

Methods are provided for altering plant characteristics by introducing into plants, isolated nucleic acid molecules that can be used to produce transgenic plants characterized by altered oil levels within the seed. Also provided are isolated nucleic acids that encode AP2 domain transcription factor proteins and fragments thereof, vectors capable of expressing such nucleic acid molecules, host cells containing such vectors, and polypeptides encoded by such nucleic acids.

ODP1-2 genes and uses thereof in plants

Methods of producing maize plants having a high oil phenotype by detecting a marker locus genetically linked with a QTL6 region

The invention provides isolated pyruvate dehydrogenase kinase nucleic acids and their encoded polypeptides. The present invention provides methods and compositions relating to altering pyruvate dehydrogenase kinase levels in plants. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.

Polynucleotides encoding proteins involved in plant metabolism

Growing community of inventors

Johnston, IA, United States of America

Peizhong Zheng