Chinese Academy of Agricultural Sciences

Other

Feed Research Institute Chinese Academy of Agricut

Feed Research Institute, Chinese Academy of Agricu

Peilong Yang

Bin Yao

Huoqing Huang

Yaru Wang

Huiying Luo

Yingguo Bai

Rui Ma

Kun Meng

Pengjun Shi

Xia Shi

Xiaoyun Su

Canfang Niu

Yangyang Li

Hong Yang

Wei Xia

Chao Shao

Heng Zhao

Tiezheng Yuan

Xiuyun Shi

Yongkai Du

Dali Yu

The present invention relates to the field of genetic engineering, particularly to phytase variant YeAPPA having improved pepsin resistance and acid resistance, and increased catalytic efficiency, by substituting Leucine at the 162site of the sequence set forth in SEQ ID NO.1 with glycine or proline or substituting glutamic acid at the 230site of the sequence set forth in SEQ ID NO.1 with glycine, proline or arginine, in the benefit of the development of economical feed enzyme industry.

Phytase variants YeAPPA having improved gastric protein resistance and acid resistance, and increased catalytic efficiency

The present invention relates to the field of genetic engineering, particularly to phytase variants YkAPPA having amino acid sequence substituting Leucine at the 162site of the sequence set forth in SEQ ID NO.1 with glycine or proline, or having amino acid sequence substituting glutamic acid at the 230site of the sequence set forth in SEQ ID NO.1 with glycine, alanine, serine, threonine, aspartic acid, proline, or arginine, and having improved pepsin resistance and increased catalytic efficiency of 2.1 times of that of the wild phytase, in the benefit of the development of economical feed enzyme industry.

Phytase variants YkAPPA having improved pepsin resistance and increased catalytic efficiency

Provided are a fungus-sourced high-temperature neutral Family-45 cullulase as well as a coding gene and application thereof. The cullulase has optimal pH value of 5.5, and optimal temperature of 60&#xb0; C., has certain enzyme activity in alkaline condition, and has good alkali resistance, maintains about 70% enzyme activity in optimal condition after being processed at 90&#xb0; C. for 1 hour, maintains about 50% enzyme activity in optimal condition after being processed in boiling water for 1 hour, and can be well applied in c and other fields.

High-temperature neutral cellulase as well as coding gene and application thereof

Provided are a fungus-sourced high-temperature acid &#x3b2;-glucosidase as well as a coding gene, and an application thereof. The provide &#x3b2;-glucosidase has the optimal pH value of 4.5 and the optimal temperature of 75&#xb0; C., and maintains over 90% enzyme activity in the optimal condition after being processed at 60&#xb0; C. for 1 h. The re-engineering yeast strain GS115/bgl3A of the coding gene comprising the &#x3b2;-glucosidase has high fermentation level.

Fungus-sourced high-temperature acid B-glucosidase as well as coding gene and application thereof

The present invention relates to the field of genetic engineering, in particular, the present invention relates to a method for producing a phytase variant with an improved thermal stability, and a phytase variant and the use thereof. The phytase variant contains at least one proline modification, compared to the phytase fromand other mutants thereof. The phytase variants with the modification have preferably improved properties, such as the thermal stability, optimal reaction temperature, pH property, specific activity, protease resistance and performance in animal feeds.

Method for producing a phytase variant with improved thermal stability, and a phytase variant and the use thereof

The present invention relates to a novel phytase enzyme, a novel isolated nucleic acid molecule coding the enzyme, and a novelhaving phytase activity. Particularly, the present invention relates to the phytase having (a) Theoretical molecular weight 45.5 kDa, (b) high specific activity 3960&#xb1;248 U/mg, (c) high stability at high temperature and wide pH, (d) optimal pH of 4.0-5.0, (e) optimal temperature of 50-60&#xb0; C., (f) high resistance to pepsin and trypsin. The phytase is very suitable to be used in feed of monogastrics as feed additive. The present invention also relates to a recombinant vector comprising said nucleic acid molecule, a recombinant host cell (e.g.,) harboring said recombinant vector, and a method for producing phytase using the recombinant host cell. The present invention further provides a feed additive comprising said phytase and/or host cells expressing a phytase as effective ingredient. In addition, the present invention provides a novel method for isolating phytase from a target organism.

Cloning and expression of a novel phytase

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Beijing, China

Peilong Yang