Jiangnan University

Peifeng Duan

Yu Liu

Xian Zhang

Zhiming Rao

Meijuan Xu

Taowei Yang

Minglong Shao

Jianing Zhang

Ziwei Wang

Yan Chen

Qi Chen

The disclosure relates to a sucrose phosphorylase mutant with improved enzyme activity, and construction method thereof and use thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the mutant of the disclosure is as shown in SEQ ID NO: 1. The mutant of the disclosure is based on sucrose phosphorylase derived from, and subjected to site-directed mutagenesis to improve the enzyme activity of sucrose phosphorylase. The mutant is expressed inand used as a whole cell catalyst to produce 2-O-&#x3b1;-D-glycerol glucoside. At a 5 L fermentation tank level, a large quantity of 2-O-&#x3b1;-D-glycerol glucoside can be produced efficiently in a short time, which is conducive to expanding the prospect of industrial application of sucrose phosphorylase for the production of 2-O-&#x3b1;-D-glycerol glucoside and realizing its large-scale industrial application.

Sucrose phosphorylase mutant with improved enzyme activity and construction method thereof and use thereof

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Wuxi, China

Peifeng Duan