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Syngenta Crop Protection Ag

Pei Su

Kasimalai Azhakanandam

Michele Susan Yarnall

Kasimalai Azhakanandamn

Rachel Whinna

Compositions and methods for transiently expressing proteins in a plant are provided. The compositions comprise plants, seeds, plant tissues, and plant parts expressing a protein, wherein the protein is expressed transiently and the transient expression of the protein can be used as a predictive model of how said protein will be expressed in stable transgenic plants in regards to qualitative and quantitative data. The predictive model may be used but is not limited to: promoter evaluation, evaluation of expression cassette construction for best performance (e.g. addition of enhancers or gene silencing suppressors), evaluation of best ways to express heterologous genes.

Method for the transient expression of nucleic acids in plants

This invention provides methods of transiently expressing a nucleotide sequence in one or more cells of a soybean plant, comprising: a) abrading the surface of a soybean plant and/or intact part thereof; and either (b1) immersing the abraded plant and/or abraded intact part thereof in a solution comprisingcells and a surfactant, wherein thecells comprise the nucleotide sequence to be transiently expressed; (b2) applying a vacuum to the immersed soybean plant and/or intact part thereof of step (b1); and (b3) releasing the vacuum, thereby introducing the solution into the plant and/or intact part thereof; or (c) contacting the abraded plant and/or abraded intact part thereof with a solution comprisingcells and a surfactant, wherein thecells comprise the nucleotide sequence to be transiently expressed, whereby the nucleotide sequence is transiently expressed in one or more cells of the plant.

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Research Triangle Park, NC, United States of America

Pei Su