Food Industry Research and Development Institute

Pei-Ming Wang

Gwo-Fang Yuan

Chii-Cherng Liao

Ting-Kuo Huang

Wen-Teng Wu

Chuen-Yang Yeh

Chin-Hang Shu

The invention discloses a method for cultivation of filamentous fungi using a suspended nutritionally solid substrate, the method including the steps of: (a) preparing a medium comprising a nutritionally solid substrate; and (b) inoculating an inoculum into the medium comprising the nutritionally solid substrate in a bioreactor to carry out fermentation. The present invention combines the properties of solid-state culture and submerged culture. In addition, the present invention may employ an air-lift bioreactor with a net draft tube and the fed-batch process to elevate the yields of filamentous fungi and metabolites thereof.

Method for cultivation of filamentous fungi

The present invention provides a process for L-phenylalanine fermentation by coryneform bacterium. The present invention successfully applies an oxystat to control the timing of feeding of molasses so as to maintain low dissolved oxygen tension and low substrate concentrations during the course of fermentation. The oxystat improves fermentation productivity appreciably. Furthermore, the present invention discovers that the proper increases of the oxygen supply rate to the culture decreases product feedback inhibition of phenylalanine formation as phenylalanine concentration increases.

Process of L-phenylalanine fermentation

Growing community of inventors

Kaohsiung, Taiwan

Pei-Ming Wang