Altius Institute for Biomedical Sciences

Pavel Zrazhevskiy

John A Stamatoyannopoulos

William Sean Kerwin

Shreeram Akilesh

Vivek Nandakumar

Alessandra Sullivan

Tobias Ragoczy

Provided herein are methods and compositions for use in detecting on-target and/or off-target cleavage of genomic DNA in a cell by a nuclease. The on-target and off-target cleavage is detected by using oligonucleotides that bind to the single-stranded 3'-overhang created at the cleavage site. The nuclease may be an engineered nuclease comprising a nucleic acid binding domain that binds to a target nucleic acid of interest.

Compositions and methods for DNA modification detection

Disclosed herein are methods of detecting a target viral nucleic acid sequence, determining the localization of the target viral nucleic acid sequence, and/or quantifying the number of target viral nucleic acid sequences in a cell. This method may be used on small target nucleic acid sequences, and may be referred to as Nano-FISH or viral Nano-FISH.

Methods and compositions for viral nano-fish

Growing community of inventors

Seattle, WA, United States of America

Pavel Zrazhevskiy