Somagenics, Inc.

Gopro, Inc.

Pavan Kumar

Brian H Johnston

Sergei A Kazakov

Bich Nguyen

David Boone

Currently, the circularization of small RNAs is broadly regarded as an obstacle in ligation-related assays and explicitly avoided while short lengths of linear RNA targets is broadly recognized as a factor limiting use of conventional primers in PCR-related assays. In contrast, the disclosed invention capitalizes on circularization of small RNA targets or their conjugates with oligonucleotide adapters. The circular RNA templates provide amplification of the target sequences via synthesis of multimer nucleic acids that can be either labeled for direct detection or subjected to PCR amplification and detection. Structure of small circular RNAs and corresponding multimeric nucleic acids provide certain advantages over current methods including flexibility in design of conventional RT and PCR primers as well as use of 5'-overlapping dimer-primers for efficient and sequence-specific amplification of short target sequences. Our invention also reduces number of steps and reagents while increasing sensitivity and accuracy of detection of small RNAs with both 2&#x2032;OH and 2&#x2032;-OMe at their 3&#x2032; ends. Our invention increase sensitivity and specificity of detection of microRNAs and other small RNAs with both 2&#x2032;OH and 2&#x2032;-OMe at their 3&#x2032; ends while allowing us to distinguish these two forms from each other.

Methods and compositions for detection of small RNAs

Timing metadata is generated and added to captured video to compensate for synchronization error between video captured concurrently from multiple cameras. A wireless beacon including timer data is transmitted from an access point to each station camera. A radio circuit of the station camera synchronizes to the timer of the access point based on timing information in the wireless beacon. An image processor in each station camera includes an image processor timer separate from the radio circuit timer. During video capture, timing metrics are generated indicating deviation between the image processor timer and the radio circuit timer. The timing metrics are stored as metadata and can be used to compensate for synchronization error in post-processing.

Synchronization of cameras using wireless beacons

Methods and compositions for detection of small RNAS

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Arlington, MA, United States of America

Pavan Kumar