Fundacao De Amparo a Pesquisa Do Estado De Sao Paulo

Fundacao De Amparo a Pesquisa Do Estado Se Sao Paulo

Fundacao De Amparo a Pesquisa Do Estado De Sao Paolo

Fundacao Oswaldo Cruz-fiocruz

Coinfair-consórcio De Indústrias Farmacêuticas

Fundaco De Amparo a Pesquisa Do Estado De Sao Paulo

Paulo Lee Ho

Ana L T O Nascimento

Elizabeth A L Martins

Luciana C C Leite

Marcia Gamberini

Miriam Tendler

Naftale Katz

Durvanei Augusto Maria

Celso Raul Romero Ramos

Ana Marisa Chudzinski-Tavassi

Isabel De Fatima Correia Batista

Isaias Raw

The present invention presents a Kunitz-type recombinant inhibitor obtained from a gene cloned from a cDNA library of salivary glands of the, and the inhibitor named Amblyomin-X has molecular mass of 13,500 Da.

Kunitz-type recombinant inhibitor

The primary objective of the present invention is the development of new mutant forms of the Sm14 protein, for producing a greater production volume. The recombinant proteins here obtained were capable of providing protection againstandinfection. The level of protection of Sm14 recombinant proteins obtained in the present invention was similar to that reached in the parasite saline extract. The mutant proteins of the present invention have reached approximately 100% of renaturation after the heating at 80&#xb0; C., different from wild forms of the Sm14 protein. Moreover, after storage for 2 months at 4&#xb0; C., mutant proteins have shown smaller &#x3b2;-structure loss than wild forms that have shown formation with random structure, as demonstrated by the circular dichroism analysis, indicating the success of mutations.

Helminth-derived antigens having capacity of providing protection against parasites

The invention relates to Leptospiral surface proteins, and the nucleic acid molecules which encode them. Various uses are described, including immunoprophylactic, diagnostic and therapeutic methods.

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São Paulo, Brazil

Paulo Lee Ho