Biofidelity Ltd

Paulina Powalowska

Barnaby Balmforth

Magdalena Stolarek-Januszkiewicz

Ana Silva-Weatherley

Methods of detecting target polynucleotide sequences may include introducing one or more nucleic acid analytes to a first reaction mixture comprising a single-stranded probe oligonucleotide A, a pyrophosphorolysing enzyme, and a ligase. The analyte may anneal to the single-stranded probe oligonucleotide Ato create a first intermediate product which is at least partially double-stranded, where the 3' end of Aforms a double-stranded complex with the analyte and where Ais pyrophosphorylsed in the 3&#x2032;-5&#x2032; direction from the 3&#x2032; end to create at least a partially digested strand A. Amay undergo ligation to form oligonucleotide A. The methods may also include detecting a signal derived from the formed oligonucleotides, and inferring therefrom the presence or absence of the target polynucleotide sequence in the analyte.

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Cambridge, United Kingdom

Paulina Powalowska