International Minerals & Chemical Corp.

Other

Paula M Keith

Wendy J Cain

Sergey A Dryga

Robert E Johnston

Nancy L Davis

Ronald I Swanstrom

Robert A Olmsted

Ian Caley

Maureen Maughan

The present invention provides methods and compositions comprising a population of alphavirus replicon particles comprising two or more isolated nucleic acids selected from 1) an isolated nucleic acid encoding an env gene product or an immunogenic fragment thereof of a human immunodeficiency virus, 2) an isolated nucleic acid encoding a g&agr;g gene product or an immunogenic fragment thereof of a human immunodeficiency virus, wherein the g&agr;g gene product or immunogenic fragment thereof is modified to inhibit formation of virus-like particles containing the g&agr;g gene product or the immunogenic fragment thereof and their release from a cell, and 3) an isolated nucleic acid encoding a pol gene product or an immunogenic fragment thereof of a human immunodeficiency virus, wherein the pol gene product or immunogenic fragment thereof is modified to inhibit protease, integrase, RNase H and/or reverse transcriptase activity, and wherein the nucleic acids are each contained within a separate alphavirus replicon particle.

Alphavirus vectors and virosomes with modified HIV genes for use in vaccines

A high titer fermentation process using transformed Escherichia coli cells carrying a plasmid containing DNA which codes for Somatomedin C is disclosed. Titers of about 900-1000 mg/L have been obtained using the method of the present invention; about two times the titer of prior art processes.

High titer production of human somatomedin C

A high density fermentation process for high yield production of bovine growth hormone by transformant E. coli is described. The process employs transformant strains of E. coli containing an expression vector coding for bovine growth hormone under the control of a bacteriophage lambda promoter-operator and a plasmid containing the .lambda.cI857 gene which codes for the temperature-sensitive repressor protein. In the initial growth period, the level of dissolved oxygen in the fermentation medium is maintained at about 20% to 60% saturation and the temperature of the medium is kept at 26.degree.-30.degree. C. Production of bovine growth hormone is then induced by raising the temperature of the medium to at least about 42.degree. C. The temperature is then reduced to about 38.degree. C. to 41.degree. C. to optimize cell growth for the remainder of the induction period, during which the level of dissolved oxygen in the medium is maintained at about 10% to 40% saturation.

Fermemtation process for the high level production of bovine growth

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Terre Haute, IN, United States of America

Paula M Keith