Pacific Biosciences of California, Inc.

Bio-rad Laboratories, Inc.

10x Genomics, Inc.

Paul Wyatt

Kevin D Ness

Phillip Belgrader

Benjamin J Hindson

Serge Saxonov

Pranav Patel

Keith P Bjornson

Paul Hardenbol

Michael Ybarra Lucero

Kamila Belhocine

Congcong Ma

Jeffrey Mellen

Nicholas Heredia

Luz Montesclaros

Indira Wu

Billy W Colston

Shawn Paul Hodges

Camille Bodley Troup

The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.

Methods and compositions for detecting genetic material

The present invention provides compositions and methods for reducing steric hindrance in the product of nucleic acid polymerase reaction. Methods and compositions of the invention encompass application of exonucleases, endonucleases, and uracil-DNA glycosylases to a nucleic acid polymerase reaction such that newly formed nucleic acid strands are modified (e.g., cleaved) while the polymerase reaction continues to proceed.

Reaction mixtures for prevention and alleviation of steric hindrance during single molecule synthesis

Prevention and alleviation of steric hindrance during single molecule nucleic acid synthesis by a polymerase

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Foster City, CA, United States of America

Paul Wyatt