10x Genomics, Inc.

Paul William Wyatt

Benjamin J Hindson

Pranav Patel

Keith P Bjornson

Paul Hardenbol

Indira Wu

Kamila Belhocine

Zahra Kamila Belhocine

This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.

Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same

Growing community of inventors

Pleasanton, CA, United States of America

Paul William Wyatt