American Cyanamid Company

Paul Wai Mak

Sotirios K Karathanasis

Bryan M O'Hara

Methods of screening for modulators of respiratory syncytial virus matrix protein interaction are described. A host cell carrying a nucleic acid sequence encoding RSV matrix protein or fragments of RSV matrix protein which can bind to RSV matrix protein is cultured and the RSV matrix protein or RSV fragments are expressed. The interaction of the expressed RSV matrix protein or fragments is measured. A test sample is then added to the expressed RSV matrix protein or fragments and the effect of the test sample on RSV matrix protein interaction is measured.

Methods of screening for modulators of respiratory syncytial virus

A method is described for screening retinoid X receptor agonists or antagonists using a retinoid X receptor expressed by a yeast expression system to screen a compound having a retinoid X receptor agonist or antagonist activity. Another screen is described for detecting a compound having retinoid X receptor agonist or antagonist activity by: (1) providing a yeast strain which expresses the retinoic acid receptor and activates a reporter plasmid containing apolipoprotein AI gene site A or a mutated variant thereof; (2) incubating the compound in suitable media and a colorless chromogenic substrate; and (3) examining the media for development of color. Mutated variants of apolipoprotein AI gene site A which respond selectively to the retinoid X receptor or respond to receptors other than RXR.alpha. are also described.

Mechanism-based screen for retinoid X receptor agonists and antagonists

Mechanism based screen for retinoid X receptor agonists and antagonists

The present invention describes a novel method for screening retinoid X receptor agonists or antagonists, comprising: using a retinoid X receptor expressed by a yeast expression system to screen a compound having a retinoid X receptor agonist or antagonist activity as well as a screen for detecting a compound having retinoid X receptor agonist or antagonist activity, which comprises the steps of (1) providing a yeast strain which expresses the retinoic acid receptor and activates a reporter plasmid containing apolipoprotein AI gene site A or a mutated variant thereof; (2) incubating the compound in suitable media and a colorless chromogenic substrate; and (3) examining the media for development of color.

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Pomona, NY, United States of America

Paul Wai Mak