Institut Pasteur

Institut National De La Sante Et De La Recherche Medicale

Paul Tchen

Patrick Grimont

Philip Kourilsky

Anne B Cami

Nicole Desplaces

Marc Leng

Francine (maiden Grimont

A method and kit for detecting a predetermined nucleotide sequence in a specimen using a nucleic acid probe modified with N-2-acetylaminofluorene (AAF).

Probe containing a modified nucleic acid recognizable by specific

The invention relates to a kit for detecting the presence of a nucleic acid sequence, such as a gene or a gene fragment, in a composition or a specimen supposed to contain it. The kit comprises a probe containing a nucleic acid complementary with the nucleic acid sequence or gene which is sought. The probe bears at least one 7-iodo-N-2-acetylamino-fluorene group covalently fixed at one at least of the bases of this probe.

Probe containing a modified nucleic acid, recognizable by specific

The invention concerns a composition of fragments of DNA utilizable as a probe for the detection of specific micoorganism, notably Legionella, in environments which contain them. These compositions are obtained from the whole genomes of the microorganisms to be detected, after fragmentation of these genomes, particularly by using a restriction enzyme, and eliminating those sequences of the genomic DNA which are susceptible to being transcribed to ribosomal RNA.

Probe and a process for the detection of specific microorganisms,

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Nanterre, France

Paul Tchen