Arcis Biotechnology Holdings Limited

Paul Reeves

Jan Rogers

Carlos Toro Rueda

A method of determining of the presence or absence of a target RNA component in a sample, the method comprising steps of: (a) contacting the sample with a composition comprising (i) a quaternary ammonium compound or a precursor thereof, which should extract/release the nucleic acid from the sample; (b) optionally storing and/or transporting the composition obtained in step (a); (c) contacting the composition obtained in step (a) with a composition comprising a proteinaceous washing agent, e.g. BSA or casein for stabilizing the RNA for transport and/or storage; and (d) using the composition obtained in step (c) in an amplification method, e.g. RT-PCR.

Method and composition using a quaternary ammonium compound

A method (e.g. high resolution melting analysis) of determining of the presence or absence of a target nucleic acid in a sample, e.g. a biological sample, the method comprising steps of:&#x22c5;(a) contacting the sample with a composition comprising&#x22c5;(i) a quaternary ammonium compound, e.g. 3-(trimethoxysilyl) propyl-dimethyl octadecyl ammonium chloride (3-TPAC) or a precursor thereof, in order to release the nucleic acids from said sample; and&#x22c5;(b) contacting the sample with a probe which is capable of interacting with the nucleic acid sequence; and&#x22c5;(c) observing and/or measuring a property of the sample, e.g. fluorescence from a dsDNA binding dye.

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Liverpool, United Kingdom

Paul Reeves