California Institute of Technology

Paul R Mueller

William G Dunphy

Akiko Kumagai

Thomas R Coleman

Barbara J Wold

Paul A Garrity

The present invention provides a threonine/tyrosine kinase, Myt1, that phosphorylates the cyclin-dependent kinase, Cdc2, to provide regulation of the cell cycle at the G2/M interphase. Also included are polynucleotides encoding Myt1 polypeptide and antibodies that bind to Myt1. Methods of modulating Myt1 for preventing premature entry of the cell into mitosis or to accelerate entry into mitosis are also provided, as are methods for identifying agents that modulate Myt1.

Method for identifying a reagent that modulates Myt1 activity

Isolated Myt1 polypeptide

DNA encoding a Myt1 polypeptide

A method for amplifying specific nucleic acid fragments having an undefined nucleotide sequence wherein said nucleic acid fragments are present in a mixture of nucleic acids. Blunt ended linkers with known nucleotide sequences are used in the method to isolate and distinguish the specific undefined nucleic acid fragments from the rest of the nucleic acid. Primers which hybridize with the known linker are then used as part of a polymerase chain reaction procedure to amplify the specific nucleic acid fragment which includes the undefined nucleotide sequence.

Method of preparing nucleic acids having an undefined nucleotide

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Chicago, IL, United States of America

Paul R Mueller