Promega Corporation

Paul Otto

Lance P Encell

Gediminas Vidugiris

Keith V Wood

Monika G Wood

Kristopher Zimmerman

Rachel Friedman Ohana

Mary P Hall

Brock Binkowski

Kris Zimmerman

Michael R Slater

Aldis Darzins

Braeden L Butler

Frank Fan

Susan Wigdal

Kate Qin Zhao

Brad Hook

Thomas A Kirkland

Dieter Klaubert

Poncho Meisenheimer

Carolyn Woodroofe Hitko

Nidhi Nath

Harry Tetsuo Uyeda

Marjeta Urh

Robin Hurst

Ji Zhu

Pete Stecha

James Robert Hartnett

Julia E Krueger

Fen Huang

Douglas R Storts

Steven T Krueger

Katharine M Miller

Alexander Novikov

Elena Bolchakova

Galina A Velikodvorskaya

Kimberly Risma

Braeden Butler

Aldis Darzin

A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-typeluciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-typeluciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-typeluciferase.

Syntheticluciferases with enhanced light output

A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-typeluciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-typeluciferase of SEQ ID NO:1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-typeluciferase.

Synthetic oplophorus luciferases with enhanced light output

The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

Compositions and methods for capture of cellular targets of bioactive agents

The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.

Mutant hydrolase proteins with enhanced kinetics and functional expression

The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture dimers), surfaces (e.g., displaying, capture ligands, capture proteins, or capture dimers), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

A modified luciferase protein which is a sensor for molecules including cAMP is provided. The modified luciferase protein includes one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with cAMP.

Luciferase biosensors for cAMP

Mutant dehalogenase proteins

[object Object]

A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule.

Mutant protease biosensors with enhanced detection characteristics

Polynucleotides encoding mutant hydrolase proteins with enhanced kinetics and functional expression

Growing community of inventors

Madison, WI, United States of America

Paul Otto